UbcH7/UBE2L3 Antibody - BSA Free

Western Blot: UbcH7/UBE2L3 Antibody [NBP1-30995] - Mouse tissue extract (50 ug) was separated by 15% SDS-PAGE, and the membrane was blotted with UbcH7/UBE2L3 antibody.

Immunocytochemistry/Immunofluorescence: UbcH7/UBE2L3 Antibody [NBP1-30995] - Analysis of HeLa, using NBP1-30995 at 1:200 dilution.

Immunohistochemistry-Paraffin: UbcH7/UBE2L3 Antibody [NBP1-30995] - A549 xenograft, using UBE2L3 antibody at 1:100 dilution. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.

Western Blot: UbcH7/UBE2L3 Antibody [NBP1-30995] - A. 30 ug Neuro2A whole cell lysate/extract, B. 30 ug GL261 whole cell lysate/extract, C. 30 ug C8D30 whole cell lysate/extract 15 % SDS-PAGE gel, antibody dilution 1:1000.

Western Blot: UbcH7/UBE2L3 Antibody [NBP1-30995] - Sample (30 ug of whole cell lysate) A: Hela B: Hep G2 12% SDS PAGE; antibody diluted at 1:1000.

Western Blot: UbcH7/UBE2L3 Antibody [NBP1-30995] - Whole zebrafish extract (30 ug) was separated by 15% SDS-PAGE, and the membrane was blotted with UbcH7/UBE2L3 antibody.

Immunoprecipitation: UbcH7/UBE2L3 Antibody [NBP1-30995] - Immunoprecipitation of UbcH7/UBE2L3 protein from HeLa whole cell extracts using 5 ug of UbcH7/UBE2L3 antibody (NBP1-30995).Western blot analysis was performed using UbcH7/UBE2L3 antibody (NBP1-30995).EasyBlot anti-Rabbit IgG was used as a secondary reagent.

• Reactivity: Hu, Mu, Ze, Po, Bv, Ch, RM, Xp
• Applications: WB, ICC/IF, IHC, IP
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

UbcH7/UBE2L3 Antibody - BSA Free Summary

• Description: Novus Biologicals Rabbit UbcH7/UBE2L3 Antibody - BSA Free (NBP1-30995) is a polyclonal antibody validated for use in IHC, WB, ICC/IF and IP. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human UbcH7/UBE2L3. The exact sequence is proprietary.
• Predicted Species: Porcine (100%), Bovine (100%), Rhesus Macaque (100%), Xenopus (100%), Chicken (100%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: UBE2L3
• Purity: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:100-1:1000
  • Immunohistochemistry 1:100-1:1000
  • Immunohistochemistry-Paraffin 1:100-1:1000
  • Immunoprecipitation 1:100-1:500
  • Western Blot 1:500-1:3000
• Theoretical MW: 18 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Xenopus laevis (100%) and Xenopus Tropicalis (100%).

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS, 40% Glycerol
• Preservative: 0.01% Thimerosal
• Purity: Antigen Affinity-purified

Alternate Names for UbcH7/UBE2L3 Antibody - BSA Free

• E2-F1
• EC 6.3.2.19
• L-UBC
• UBCE7
• UbcH7
• UBCH7UbcM4
• UbcM4
• UBE2L3
• Ubiquitin carrier protein L3
• ubiquitin-conjugating enzyme E2 L3
• Ubiquitin-conjugating enzyme E2-F1
• ubiquitin-conjugating enzyme E2L 3
• ubiquitin-conjugating enzyme UBCH7
• Ubiquitin-protein ligase L3

Background

The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes (E1s), ubiquitin-conjugating enzymes (E2s) and ubiquitin-protein ligases (E3s). This gene encodes a member of the E2 ubiquitin-conjugating enzyme family. This enzyme is demonstrated to participate in the ubiquitination of p53, c-Fos, and the NF-kB precursor p105 in vitro. Two alternatively spliced transcript variants encoding distinct isoforms have been found for this gene.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

Bioinformatics

• Gene Symbol: UBE2L3