UbcH7/UBE2L3 Antibody (3B7) - Azide and BSA Free

Western Blot: UbcH7/UBE2L3 Antibody (3B7) [H00007332-M01] - UBE2L3 monoclonal antibody (M01), clone 3B7 Analysis of UBE2L3 expression in K-562.

Immunocytochemistry/Immunofluorescence: UbcH7/UBE2L3 Antibody (3B7) [H00007332-M01] - Analysis of monoclonal antibody to UBE2L3 on HeLa cell. Antibody concentration 10 ug/ml.

• Reactivity: Hu
• Applications: WB, ELISA, ICC/IF
• Clone: 3B7
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free

UbcH7/UBE2L3 Antibody (3B7) - Azide and BSA Free Summary

• Description: Quality control test: Antibody Reactive Against Recombinant Protein.
• Immunogen: UBE2L3 (AAH53368, 1 a.a. ~ 154 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. MAASRRLMKELEEIRKCGMKNFRNIQVDEANLLTWQGLIVPDNPPYDKGAFRIEINFPAEYPFKPPKITFKTKIYHPNIDEKGQVCLPVISAENWKPATKTDQVIQSLIALVNDPQPEHPLRADLAEEYSKDRKKFCKNAEEFTKKYGEKRPVD
• Specificity: UBE2L3 - ubiquitin-conjugating enzyme E2L 3
• Isotype: IgG2a Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: UBE2L3
• Purity: IgG purified

Applications/Dilutions

• Dilutions:
  • ELISA
  • Immunocytochemistry/ Immunofluorescence
  • Western Blot 1:500
• Application Notes: Antibody reactivity against cell lysate and recombinant protein for WB. It has also been used for IF and ELISA.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: In 1x PBS, pH 7.4
• Preservative: No Preservative
• Purity: IgG purified

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for UbcH7/UBE2L3 Antibody (3B7) - Azide and BSA Free

• E2-F1
• EC 6.3.2.19
• L-UBC
• UBCE7
• UbcH7
• UBCH7UbcM4
• UbcM4
• UBE2L3
• Ubiquitin carrier protein L3
• ubiquitin-conjugating enzyme E2 L3
• Ubiquitin-conjugating enzyme E2-F1
• ubiquitin-conjugating enzyme E2L 3
• ubiquitin-conjugating enzyme UBCH7
• Ubiquitin-protein ligase L3

Background

The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes (E1s), ubiquitin-conjugating enzymes (E2s) and ubiquitin-protein ligases (E3s). This gene encodes a member of the E2 ubiquitin-conjugating enzyme family. This enzyme is demonstrated to participate in the ubiquitination of p53, c-Fos, and the NF-kB precursor p105 in vitro. Two alternatively spliced transcript variants encoding distinct isoforms have been found for this gene.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: UBE2L3
• Entrez:
  • 7332(Human)
• OMIM:
  • 603721(Human)
• Uniprot:
  • AAH53368(Human)