UbcH5a/UBE2D1 Antibody - BSA Free

Western Blot: UbcH5a/UBE2D1 Antibody [NBP1-32734] - Sample (30 ug of whole cell lysate) A: A431 10% SDS PAGE, antibody diluted at 1:1000.

Immunocytochemistry/Immunofluorescence: UbcH5a/UBE2D1 Antibody [NBP1-32734] - Analysis of paraformaldehyde-fixed A431, using UBE2D1 antibody at 1:200 dilution.

Immunohistochemistry-Paraffin: UbcH5a/UBE2D1 Antibody [NBP1-32734] - Mouse lung. UBE2D1 antibody dilution: 1:500. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.

Immunohistochemistry-Paraffin: UbcH5a/UBE2D1 Antibody [NBP1-32734] - TOV-112D xenograft, using UBE2D1 antibody at 1:500 dilution. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.

Immunohistochemistry-Paraffin: UbcH5a/UBE2D1 Antibody [NBP1-32734] - Mouse prostate. UBE2D1 antibody dilution: 1:500. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.

• Reactivity: Hu, Mu, Rt, Bv, Ch, RM, Xp
• Applications: WB, ICC/IF, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

UbcH5a/UBE2D1 Antibody - BSA Free Summary

• Description: Novus Biologicals Rabbit UbcH5a/UBE2D1 Antibody - BSA Free (NBP1-32734) is a polyclonal antibody validated for use in IHC, WB and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Full length human UbcH5a/UBE2D1 Recombinant protein.
• Predicted Species: Rat (100%), Chicken (100%), Xenopus (95%), Rhesus Macaque (100%), Bovine (100%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: UBE2D1
• Purity: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:100-1:1000
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 1:100-1:1000
  • Western Blot 1:500-1:3000
• Theoretical MW: 17 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Xenopus laevis (95%).

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: 0.1M Tris, 0.1M Glycine, 10% Glycerol
• Preservative: 0.01% Thimerosal
• Purity: Antigen Affinity-purified

Alternate Names for UbcH5a/UBE2D1 Antibody - BSA Free

• E2(17)KB1
• Stimulator of Fe transportUBC4/5 homolog
• UBC4/5
• UBC5A
• UbcH5
• UbcH5a
• UBCH5AEC 6.3.2.19
• UBCH5SFT
• UBE2D1
• Ubiquitin carrier protein D1
• ubiquitin-conjugating enzyme E2 D1
• Ubiquitin-conjugating enzyme E2(17)KB 1
• Ubiquitin-conjugating enzyme E2-17 kDa 1
• ubiquitin-conjugating enzyme E2D 1 (UBC4/5 homolog, yeast)
• Ubiquitin-protein ligase D1

Background

The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes, or E1s, ubiquitin-conjugating enzymes, or E2s, and ubiquitin-protein ligases, or E3s. This gene encodes a member of the E2 ubiquitin-conjugating enzyme family. This enzyme is closely related to a stimulator of iron transport (SFT), and is up-regulated in hereditary hemochromatosis. It also functions in the ubiquitination of the tumor-suppressor protein p53 and the hypoxia-inducible transcription factor HIF1alpha by interacting with the E1 ubiquitin-activating enzyme and the E3 ubiquitin-protein ligases.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

Bioinformatics

• Gene Symbol: UBE2D1