UbcH5a/UBE2D1 Antibody

Images

 
Western Blot: UbcH5a/UBE2D1 Antibody [NBP1-32734] - Sample (30 ug of whole cell lysate) A: A431 10% SDS PAGE, antibody diluted at 1:1000.
Immunohistochemistry-Paraffin: UbcH5a/UBE2D1 Antibody [NBP1-32734] - Paraffin-embedded TOV-112D xenograft, using antibody at 1:500 dilution.

Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications WB, ICC/IF, IHC, IHC-P
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1 mg/ml

Order Details

UbcH5a/UBE2D1 Antibody Summary

Immunogen
Full length human UBE2D1 Recombinant protein.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
UBE2D1
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:500-1:3000
  • Immunocytochemistry/Immunofluorescence 1:100-1:1000
  • Immunohistochemistry 10 - 1:500
  • Immunohistochemistry-Paraffin 1:100-1:1000

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
0.1M Tris (pH 7.0), 0.1M Glycine and 10% Glycerol
Preservative
0.01% Thimerosal
Concentration
1 mg/ml
Purity
Immunogen affinity purified

Alternate Names for UbcH5a/UBE2D1 Antibody

  • E2(17)KB1
  • Stimulator of Fe transportUBC4/5 homolog
  • UBC4/5
  • UBC5A
  • UbcH5
  • UbcH5a
  • UBCH5AEC 6.3.2.19
  • UBCH5SFT
  • UBE2D1
  • Ubiquitin carrier protein D1
  • ubiquitin-conjugating enzyme E2 D1
  • Ubiquitin-conjugating enzyme E2(17)KB 1
  • Ubiquitin-conjugating enzyme E2-17 kDa 1
  • ubiquitin-conjugating enzyme E2D 1 (UBC4/5 homolog, yeast)
  • Ubiquitin-protein ligase D1

Background

The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes, or E1s, ubiquitin-conjugating enzymes, or E2s, and ubiquitin-protein ligases, or E3s. This gene encodes a member of the E2 ubiquitin-conjugating enzyme family. This enzyme is closely related to a stimulator of iron transport (SFT), and is up-regulated in hereditary hemochromatosis. It also functions in the ubiquitination of the tumor-suppressor protein p53 and the hypoxia-inducible transcription factor HIF1alpha by interacting with the E1 ubiquitin-activating enzyme and the E3 ubiquitin-protein ligases.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Secondary Antibodies

 

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Bioinformatics Tool for UbcH5a/UBE2D1 Antibody (NBP1-32734)

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Bioinformatics

Gene Symbol UBE2D1
Entrez