UBAP2L Antibody

Western Blot: UBAP2L Antibody [NB100-750] - Detection of human UBAP2L/NICE-4 by western blot. Samples: Whole cell lysate (10 ug) from HEK293T, HeLa, Jurkat, K-562, and MCF-7 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-UBAP2L/NICE-4 antibody NB100-750 used for WB at 0.04 ug/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.

Western Blot: UBAP2L Antibody [NB100-750] - Whole cell lysate (100 ug) from HeLa cells either mock treated, treated with a control siRNA, or treated with siRNA for UBAP2L/NICE4. Antibody used at 0.03 ug/ml.

Western Blot: UBAP2L Antibody [NB100-750] - Detection of Human UBAP2L/NICE4 by Western Blot. Samples: Whole cell lysate (50 ug) from HeLa, 293T, and Jurkat cells. Antibodies: Affinity purified rabbit anti-UBAP2L/NICE4 antibody NB100-750 used for WB at 0.04 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.

• Reactivity: Hu
• Applications: WB, IP, KD
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Concentration: 1.0 mg/ml

UBAP2L Antibody Summary

• Immunogen: The immunogen recognized by this antibody maps to a region between residues 400 and 450 of human Ubiquitin Associated Protein 2-Like using the numbering given in entry AAH03170.1 (GeneID 9898).
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: UBAP2L
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunoprecipitation 2 - 10 ug/mg lysate
  • Knockdown Validated
  • Western Blot 1:5000 - 1:25000
• Control:

  UBAP2L Overexpression Lysate

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: Tris-Citrate/Phosphate (pH 7.0 - 8.0)
• Preservative: 0.09% Sodium Azide
• Concentration: 1.0 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for UBAP2L Antibody

• FLJ42300
• KIAA0144NICE-4
• NICE4
• Protein NICE-4
• ubiquitin associated protein 2-like
• ubiquitin-associated protein 2-like

Background

Ubiquitin associated protein 2-like (UBAP2L) has been identified as the predicted gene product of a coding sequence deduced by analysis of cDNAs from a human myeloid cell line. UBAP2L was also identified as a cDNA belonging to the epidermal differentiation complex (EDC) which comprises a large number of genes important to human epidermis maturation. A function of UBAP2L has not been assigned. UBAP2L is also known as NICE4, FLJ42300 and KIAA0144.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: UBAP2L
• Entrez:
  • 9898(Human)
• Uniprot:
  • AAH03170.1(Human)