UBA3 Antibody

Western Blot: UBA3 Antibody [NBP2-75926] - analysis of UBE1C on human liver tissue lysate using anti-UBE1C antibody at 1/200 dilution.

Immunocytochemistry/Immunofluorescence: UBA3 Antibody [NBP2-75926] - staining UBE1C in SH-SY5Y cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Immunohistochemistry-Paraffin: UBA3 Antibody [NBP2-75926] - analysis of paraffin-embedded mouse testis tissue using anti-UBE1C antibody. Counter stained with hematoxylin

Flow Cytometry: UBA3 Antibody [NBP2-75926] - analysis of HUVEC cells with UBE1C antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; green).

Immunocytochemistry/Immunofluorescence: UBA3 Antibody [NBP2-75926] - staining UBE1C in A431 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Immunocytochemistry/Immunofluorescence: UBA3 Antibody [NBP2-75926] - staining UBE1C in HUVEC cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Immunohistochemistry-Paraffin: UBA3 Antibody [NBP2-75926] - analysis of paraffin-embedded rat brain tissue using anti-UBE1C antibody. Counter stained with hematoxylin.

Immunohistochemistry-Paraffin: UBA3 Antibody [NBP2-75926] - analysis of paraffin-embedded human pancreas tissue using anti-UBE1C antibody. Counter stained with hematoxylin.

Product Discontinued

UBA3 Antibody Summary

• Immunogen: Recombinant protein within Human UBA3 aa 220-424 / 463. (SwissProt: Q8TBC4 Human; SwissProt: Q8C878 Mouse; SwissProt: Q99MI7 Rat)
• Localization: Nucleus. Cytoplasm.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: UBA3
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 1:50-1:100
  • Immunocytochemistry/ Immunofluorescence 1:50-1:200
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 1:50-1:200
  • Western Blot 1:500
• Theoretical MW: 52 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS (pH7.4), 0.2% BSA, 50% Glycerol
• Preservative: 0.05% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Protein A purified

Alternate Names for UBA3 Antibody

• DKFZp566J164
• EC 6.3.2
• EC 6.3.2.-
• hUBA3
• MGC22384
• NEDD8-activating enzyme E1 catalytic subunit
• NEDD8-activating enzyme E1C
• Nedd8-activating enzyme hUba3
• UBA3
• UBA3, ubiquitin-activating enzyme E1 homolog
• UBE1C
• Ubiquitin-activating enzyme 3
• ubiquitin-activating enzyme E1C (homologous to yeast UBA3)
• Ubiquitin-activating enzyme E1C
• ubiquitin-like modifier activating enzyme 3
• Ubiquitin-like modifier-activating enzyme 3
• yeast)

Background

The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes, or E1s, ubiquitin-conjugating enzymes, or E2s, and ubiquitin-protein ligases, or E3s. This gene encodes a member of the E1 ubiquitin-activating enzyme family. The encoded enzyme associates with AppBp1, an amyloid beta precursor protein binding protein, to form a heterodimer, and then the enzyme complex activates NEDD8, a ubiquitin-like protein, which regulates cell division, signaling and embryogenesis. Multiple alternatively spliced transcript variants encoding distinct isoforms have been found for this gene.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: UBA3