UAP56 Antibody - BSA Free

Western Blot: UAP56 Antibody [NBP2-58594] - Analysis in human cell lines HEK293 and MCF-7. Corresponding RNA-seq data are presented for the same cell lines. Loading control: Anti-PFN1.

Immunocytochemistry/Immunofluorescence: UAP56 Antibody [NBP2-58594] - Staining of human cell line MCF7 shows localization to nuclear speckles. Antibody staining is shown in green.

Immunohistochemistry-Paraffin: UAP56 Antibody [NBP2-58594] - Staining in human epididymis and duodenum tissues using anti-DDX39B antibody. Corresponding DDX39B RNA-seq data are presented for the same tissues.

Western Blot: UAP56 Antibody [NBP2-58594] - Western blot analysis in human cell line RT-4 and human cell line U-251 MG.

Immunohistochemistry-Paraffin: UAP56 Antibody [NBP2-58594] - Staining of human duodenum shows low expression as expected.

Immunohistochemistry-Paraffin: UAP56 Antibody [NBP2-58594] - Staining of human epididymis shows high expression.

• Reactivity: Hu, Mu, Rt
• Applications: WB, ICC/IF, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

UAP56 Antibody - BSA Free Summary

• Description: Novus Biologicals Rabbit UAP56 Antibody - BSA Free (NBP2-58594) is a polyclonal antibody validated for use in IHC, WB and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: This antibody was developed against a recombinant protein corresponding to the following amino acid sequence: MAENDVDNELLDYEDDEVETAAGGD
• Predicted Species: Mouse (96%), Rat (96%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: DDX39B
• Purity: Affinity purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 0.25-2 ug/ml
  • Immunohistochemistry 1:20 - 1:50
  • Immunohistochemistry-Paraffin 1:20 - 1:50
  • Western Blot 0.04-0.4 ug/ml
• Application Notes: For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.
• Control Peptide:

  UAP56 Recombinant Protein Antigen (NBP2-58594PEP)

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.2) and 40% Glycerol
• Preservative: 0.02% Sodium Azide
• Purity: Affinity purified

Alternate Names for UAP56 Antibody - BSA Free

• 56 kDa U2AF65-associated protein
• ATP-dependent RNA helicase p47
• DEAD (Asp-Glu-Ala-Asp) box polypeptide 39B
• DEAD box protein UAP56
• EC 3.6.4.13
• HLA-B associated transcript 1
• HLA-B-associated transcript 1 protein
• spliceosome RNA helicase BAT1
• UAP56D6S81EBAT1nuclear RNA helicase (DEAD family)

Background

UAP56 encodes a member of the DEAD box family of RNA-dependent ATPases that mediate ATP hydrolysis during pre-mRNA splicing. The encoded protein is an essential splicing factor required for association of U2 small nuclear ribonucleoprotein with pre-mRNA, and also plays an important role in mRNA export from the nucleus to the cytoplasm. A cluster of genes, BAT1-BAT5, is localized in the vicinity of the genes for TNF alpha and TNF beta. These genes are all within the human major histocompatibility complex class III region. Mutations in this gene may be associated with rheumatoid arthritis. Alternatively spliced transcript variants encoding the same protein have been described.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: DDX39B