UAP56 Antibody

Western Blot: UAP56 Antibody [NBP1-31356] - UAP56 antibody detects UAP56 protein by western blot analysis. Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with UAP56 antibody diluted by 1:500. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Immunocytochemistry/Immunofluorescence: UAP56 Antibody [NBP1-31356] - UAP56 antibody [N1C2] detects UAP56 protein at nucleus by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: UAP56 protein stained by UAP56 antibody [N1C2] diluted at 1:500. Red: alpha Tubulin protein stained by alpha Tubulin antibody [GT114] diluted at 1:1000. Scale bar = 10 um.

Immunohistochemistry-Paraffin: UAP56 Antibody [NBP1-31356] - UAP56 antibody [N1C2] detects UAP56 protein at nucleus in mouse brain by immunohistochemical analysis. Sample: Paraffin-embedded mouse brain. UAP56 antibody [N1C2] diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Western Blot: UAP56 Antibody [NBP1-31356] - Sample (30 ug of whole cell lysate) A: 293T 10% SDS PAGE; antibody diluted at 1:1000.

Immunocytochemistry/Immunofluorescence: UAP56 Antibody [NBP1-31356] - Analysis of HeLa, using NBP1-31356 at 1:200 dilution.

Immunohistochemistry-Paraffin: UAP56 Antibody [NBP1-31356] - Paraffin-embedded A549 xenograft, using antibody at 1:100 dilution.

Immunohistochemistry-Paraffin: UAP56 Antibody [NBP1-31356] - UAP56 antibody [N1C2] detects UAP56 protein at nucleus in rat liver by immunohistochemical analysis. Sample: Paraffin-embedded rat liver. UAP56 antibody [N1C2] diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Immunohistochemistry-Paraffin: UAP56 Antibody [NBP1-31356] - UAP56 antibody [N1C2] detects UAP56 protein at nucleus in mouse ovary by immunohistochemical analysis. Sample: Paraffin-embedded mouse ovary. UAP56 antibody [N1C2] diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

• Reactivity: Hu, Mu, Rt, Bv, Ca, Xp, Ze
• Applications: WB, ICC/IF, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated

UAP56 Antibody Summary

• Immunogen: Recombinant protein encompassing a sequence within the center region of human UAP56. The exact sequence is proprietary.
• Localization: Nucleus
• Predicted Species: Canine (100%), Xenopus (96%), Zebrafish (92%), Bovine (99%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: DDX39B
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/Immunofluorescence 1:100-1:1000
  • Immunohistochemistry 10 - 1:500
  • Immunohistochemistry-Paraffin 1:100-1:1000
  • Western Blot 1:500-1:3000

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Xenopus laevis (96%) and Xenopus tropicalis (96%).

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: 0.1M Tris (pH 7), 0.1M Glycine, 10% Glycerol
• Preservative: 0.01% Thimerosal
• Purity: Immunogen affinity purified

Alternate Names for UAP56 Antibody

• 56 kDa U2AF65-associated protein
• ATP-dependent RNA helicase p47
• DEAD (Asp-Glu-Ala-Asp) box polypeptide 39B
• DEAD box protein UAP56
• EC 3.6.4.13
• HLA-B associated transcript 1
• HLA-B-associated transcript 1 protein
• spliceosome RNA helicase BAT1
• UAP56D6S81EBAT1nuclear RNA helicase (DEAD family)

Background

This gene encodes a member of the DEAD box family of RNA-dependent ATPases that mediate ATP hydrolysis during pre-mRNA splicing. The encoded protein is an essential splicing factor required for association of U2 small nuclear ribonucleoprotein with pre-mRNA, and also plays an important role in mRNA export from the nucleus to the cytoplasm. A cluster of genes, BAT1-BAT5, is localized in the vicinity of the genes for TNF alpha and TNF beta. These genes are all within the human major histocompatibility complex class III region. Mutations in this gene may be associated with rheumatoid arthritis. Alternatively spliced transcript variants encoding the same protein have been described.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

Bioinformatics

• Gene Symbol: DDX39B