UAP56 Antibody

Images

 
Western Blot: UAP56 Antibody [NBP1-31356] - Sample (30 ug of whole cell lysate) A: 293T 10% SDS PAGE; antibody diluted at 1:1000.
Immunocytochemistry/ Immunofluorescence: UAP56 Antibody [NBP1-31356] - Analysis of HeLa, using NBP1-31356 at 1:200 dilution.
Immunohistochemistry-Paraffin: UAP56 Antibody [NBP1-31356] - Paraffin-embedded A549 xenograft, using antibody at 1:100 dilution.

Product Details

Summary
Reactivity Hu, Mu, Rt, Bv, Ca, Xp, ZeSpecies Glossary
Applications WB, ICC/IF, IHC, IHC-P
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
0.43 mg/ml

Order Details

UAP56 Antibody Summary

Immunogen
Recombinant protein encompassing a sequence within the center region of human UAP56. The exact sequence is proprietary.
Localization
Nucleus
Predicted Species
Bovine (99%), Zebrafish (92%), Xenopus (96%), Canine (100%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
DDX39B
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:500-1:3000
  • Immunocytochemistry/Immunofluorescence 1:100-1:1000
  • Immunohistochemistry 10 - 1:500
  • Immunohistochemistry-Paraffin 1:100-1:1000

Reactivity Notes

Expected cross reactivity based on sequence homology: Xenopus laevis (96%) and Xenopus tropicalis (96%).

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
0.1M Tris (pH 7.0), 0.1M Glycine and 10% Glycerol
Preservative
0.01% Thimerosal
Concentration
0.43 mg/ml
Purity
Immunogen affinity purified

Alternate Names for UAP56 Antibody

  • 56 kDa U2AF65-associated protein
  • ATP-dependent RNA helicase p47
  • DEAD (Asp-Glu-Ala-Asp) box polypeptide 39B
  • DEAD box protein UAP56
  • EC 3.6.4.13
  • HLA-B associated transcript 1
  • HLA-B-associated transcript 1 protein
  • spliceosome RNA helicase BAT1
  • UAP56D6S81EBAT1nuclear RNA helicase (DEAD family)

Background

This gene encodes a member of the DEAD box family of RNA-dependent ATPases that mediate ATP hydrolysis during pre-mRNA splicing. The encoded protein is an essential splicing factor required for association of U2 small nuclear ribonucleoprotein with pre-mRNA, and also plays an important role in mRNA export from the nucleus to the cytoplasm. A cluster of genes, BAT1-BAT5, is localized in the vicinity of the genes for TNF alpha and TNF beta. These genes are all within the human major histocompatibility complex class III region. Mutations in this gene may be associated with rheumatoid arthritis. Alternatively spliced transcript variants encoding the same protein have been described.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol DDX39B