UAP56 Antibody (3A2B2) - BSA Free

Western Blot: UAP56 Antibody (3A2B2) [NBP2-52456] - Analysis using DDX39B mouse mAb against HEK293 (1), Jurkat (2), MCF-7 (3), A431 (4), NIH/3T3 (5), Jurkat (6), K562 (7), and HepG2 (8) cell lysate.

Immunohistochemistry-Paraffin: UAP56 Antibody (3A2B2) [NBP2-52456] - Analysis of cervical cancer tissues using DDX39B mouse mAb with DAB staining.

Western Blot: UAP56 Antibody (3A2B2) [NBP2-52456] - Analysis using DDX39B mAb against human DDX39B (AA: 1-250) recombinant protein. (Expected MW is 54.2 kDa)

Western Blot: UAP56 Antibody (3A2B2) [NBP2-52456] - Analysis using DDX39B mAb against HEK293 (1) and DDX39B (AA: 1-250)-hIgGFc transfected HEK293 (2) cell lysate.

ELISA: UAP56 Antibody (3A2B2) [NBP2-52456] - Black line: Control Antigen (100 ng); Purple line: Antigen(10ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng).

• Reactivity: Hu, Mu
• Applications: WB, ELISA, IHC, CyTOF-ready
• Clone: 3A2B2
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1 mg/ml

UAP56 Antibody (3A2B2) - BSA Free Summary

• Description: Novus Biologicals Mouse UAP56 Antibody (3A2B2) - BSA Free (NBP2-52456) is a monoclonal antibody validated for use in IHC, WB and ELISA. Anti-UAP56 Antibody: Cited in 1 publication. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Purified recombinant fragment of human UAP56 (AA: 1-250) expressed in E. Coli.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: DDX39B
• Purity: Protein G purified

Applications/Dilutions

• Dilutions:
  • CyTOF-ready
  • ELISA 1:10000
  • Immunohistochemistry 1:200-1:1000
  • Immunohistochemistry-Paraffin 1:200-1:1000
  • Western Blot 1:500-1:2000
• Application Notes: This antibody is Cytof ready.
• Theoretical MW: 49 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: 0.05% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Protein G purified

Alternate Names for UAP56 Antibody (3A2B2) - BSA Free

• 56 kDa U2AF65-associated protein
• ATP-dependent RNA helicase p47
• DEAD (Asp-Glu-Ala-Asp) box polypeptide 39B
• DEAD box protein UAP56
• EC 3.6.4.13
• HLA-B associated transcript 1
• HLA-B-associated transcript 1 protein
• spliceosome RNA helicase BAT1
• UAP56D6S81EBAT1nuclear RNA helicase (DEAD family)

Background

UAP56 encodes a member of the DEAD box family of RNA-dependent ATPases that mediate ATP hydrolysis during pre-mRNA splicing. The encoded protein is an essential splicing factor required for association of U2 small nuclear ribonucleoprotein with pre-mRNA, and also plays an important role in mRNA export from the nucleus to the cytoplasm. A cluster of genes, BAT1-BAT5, is localized in the vicinity of the genes for TNF alpha and TNF beta. These genes are all within the human major histocompatibility complex class III region. Mutations in this gene may be associated with rheumatoid arthritis. Alternatively spliced transcript variants encoding the same protein have been described.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for UAP56 Antibody (NBP2-52456)(1)

Publication using NBP2-52456

• Boehringer A, Garcia-Mansfield K, Singh G et al. ALS Associated Mutations in Matrin 3 Alter Protein-Protein Interactions and Impede mRNA Nuclear Export Sci Rep. 2017-11-06 [PMID: 29109432] (WB, Mouse)

Bioinformatics

• Gene Symbol: DDX39B