UAP1 Antibody - BSA Free

Western Blot: UAP1 Antibody [NBP1-89197] - Analysis in A-431 cells transfected with control siRNA, target specific siRNA probe #1 and #2,. Remaining relative intensity is presented. Loading control: Anti-GAPDH.

Immunocytochemistry/Immunofluorescence: UAP1 Antibody [NBP1-89197] - Staining of human cell line A-431 shows localization to nucleoplasm, plasma membrane & cytosol.

Immunohistochemistry-Paraffin: UAP1 Antibody [NBP1-89197] - Staining of human adrenal gland shows moderate cytoplasmic positivity in glandular cells.

Western Blot: UAP1 Antibody [NBP1-89197] - Analysis in human cell line U-251 MG.

Immunohistochemistry-Paraffin: UAP1 Antibody [NBP1-89197] - Staining of human testis shows cytoplasmic and nuclear positivity of cells in seminiferus ducts.

• Reactivity: Hu
• Applications: WB, ICC/IF, IHC, KD
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

UAP1 Antibody - BSA Free Summary

• Immunogen: This antibody was developed against Recombinant Protein corresponding to amino acids: NDLKLTLSKAGQEHLLRFWNELEEAQQVELYAELQAMNFEELNFFFQKAIEGFNQSSHQKNVDARMEPVPREVLGSATRDQDQLQAWESEGLFQISQNKVAVLLLAGGQGTRLGVAYPKGMYDVGLPSRKTLFQIQAERILKLQQ
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: UAP1
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 0.25-2 ug/ml
  • Immunohistochemistry 1:20 - 1:50
  • Immunohistochemistry-Paraffin 1:20 - 1:50
  • Knockdown Validated
  • Western Blot 0.04-0.4 ug/ml
• Application Notes: For IHC-Paraffin, HIER pH 6 retrieval is recommended. Immunocytochemistry/Immunofluorescence Fixation Permeabilization: Use PFA/Triton X-100.
• Control Peptide:

  UAP1 Protein (NBP1-89197PEP)

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Mouse (87%), Rat (85%). Reactivity reported in scientific literature (PMID: 23724116)

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.2) and 40% Glycerol
• Preservative: 0.02% Sodium Azide
• Purity: Immunogen affinity purified

Alternate Names for UAP1 Antibody - BSA Free

• AGX
• AGX1
• AGX2
• Antigen X
• EC 2.7.7
• EC 2.7.7.23
• EC 2.7.7.27
• SPAG2
• sperm associated antigen 2
• Sperm-associated antigen 2
• UDP-N-acetylhexosamine pyrophosphorylase
• UDP-N-acteylglucosamine pyrophosphorylase 1

Background

Converts UDP and GlcNAc-1-P into UDP-GlcNAc, and UDP and GalNAc-1-P into UDP-GalNAc. Isoform AGX1 has 2 to 3 times higher activity towards GalNAc-1-P, while isoform AGX2 has 8 times more activity towards GlcNAc-1-P

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for UAP1 Antibody (NBP1-89197)(1)

Publication using NBP1-89197

• Itkonen HM, Mills IG. N-Linked Glycosylation Supports Cross-Talk between Receptor Tyrosine Kinases and Androgen Receptor. PLoS One 2013-01-01 [PMID: 23724116] (WB)

Bioinformatics

• Gene Symbol: UAP1