u-Plasminogen Activator/Urokinase Antibody (U-16) - BSA Free

Product Discontinued

u-Plasminogen Activator/Urokinase Antibody (U-16) - BSA Free Summary

• Immunogen: Native human uPA
• Epitope: Binds to the B-chain of uPA
• Specificity: It is specific for human uPA. No reaction with human tissue plasminogen activator (tPA) when tested by ELISA, immunoblotting and enzyme inhibition and no reaction with any other human plasma proteins when tested by immunoblotting. Binds single and two-chain uPA and uPA/PAI-1 complexes. A reaction is seen with LMW-uPA but not with amino terminal fregment (ATF).
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: PLAU
• Purity: Protein A or G purified

Applications/Dilutions

• Dilutions:
  • ELISA 1:100-1:2000
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Frozen 1:10-1:500
  • Western Blot 1:100-1:2000
• Application Notes: ELISA: NBP1-05160 can be used in ELISA. (1) WB: NBP1-05160 reacts with non-reduced uPA but not with reduced uPA in Western blotting. IHC: NBP1-05160 is recommended for flowcytometry and immunoflourescence studies and can be used on frozen sections in immunohistochemistry applications.

Packaging, Storage & Formulations

• Storage: Store at 4C in the dark.
• Buffer: 0.01 M phosphate buffer, pH 7.4, containing 0.14 M NaCl
• Preservative: 0.9% Sodium Azide
• Concentration: 1.0 mg/ml
• Purity: Protein A or G purified

Alternate Names for u-Plasminogen Activator/Urokinase Antibody (U-16) - BSA Free

• ATF
• EC 3.4.21
• EC 3.4.21.73
• plasminogen activator, urokinase
• PLAU
• uPA
• u-PA
• uPlasminogen Activator
• u-Plasminogen Activator
• urinary
• Urokinase
• urokinase-type plasminogen activator

Background

Urokinase plasminogen activator (uPA) is a serine protease. It converts the abundant proenzyme plasminogen to active plasmin and plays a key role in cancer invasion and a variety of tissue remodeling processes such as wound healing, mammary gland involution and placental development (1-3). Elevated levels of uPA are associated with poor prognosis in many types of cancer (2-5).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for u-Plasminogen Activator/Urokinase Antibody (NBP1-05160)(1)

Publication using NBP1-05160

• Halbert D, Domenyuk V, Spetzler D et al. Aptamers and uses thereof United States Patent Application US 9958448 B2 2018-01-01

Bioinformatics

• Gene Symbol: PLAU