TUG Antibody - BSA Free

Western Blot: TUG Antibody [NBP3-12632] - Lane A: K562 Whole Cell LysateLane B: 293T Whole Cell LysateLane C: SW480 Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution.Developed using the ECL technique.Performed under reducing conditions.Predicted band size:60 kDaObserved band size:72 kDa

Immunohistochemistry-Paraffin: TUG Antibody [NBP3-12632] - Staining of human ASPSCR1 in human liver with rabbit polyclonal antibody at 1:1000 dilution.

Immunohistochemistry-Paraffin: TUG Antibody [NBP3-12632] - Staining of human ASPSCR1 in human kidney with rabbit polyclonal antibody at 1:1000 dilution.

Immunoprecipitation: TUG Antibody [NBP3-12632] - Lane A:0.5 mg K562 Whole Cell Lysate4 uL anti-ASPSCR1 rabbit polyclonal antibody and 60 ug of Immunomagnetic beads Protein A/G.Primary antibody:Anti-ASPSCR1 rabbit polyclonal antibody,at 1:100 dilution Secondary antibody:Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilutionDeveloped using the ECL technique.Performed under reducing conditions.Predicted band size: 60 kDaObserved band size :72 kDa

Immunocytochemistry/Immunofluorescence: TUG Antibody [NBP3-12632] - Staining of ASPSCR1 in U2OS cells. Cells were fixed with 4% PFA, permeabilzed with 0.1% Triton X-100 in PBS,blocked with 10% serum, and incubated with NBP3-12632 antibody (dilution ratio 1:200) at 4? overnight. Then cells were stained with the Alexa Fluor488-conjugated Goat Anti-rabbit IgG secondary antibody (green). Positive staining was localized to Nucleus?Cytoplasm and cell membrane.

• Reactivity: Hu
• Applications: WB, ICC/IF, IHC, IP
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

TUG Antibody - BSA Free Summary

• Description: This antibody can be stored at 2-8C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20C to -80C. Avoid repeated freeze-thaw cycles.
  
  Protein A & Antigen Affinity purified
• Immunogen: Produced in rabbits immunized with E. coli-derived Human TUG fragment
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: ASPSCR1
• Purity: Antigen and protein A Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:100-1:500
  • Immunohistochemistry-Paraffin 1:500-1:2000
  • Immunoprecipitation 1-5uL/mg of lysate
  • Western Blot 1:500-1:2000

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: 0.03% Proclin 300
• Purity: Antigen and protein A Affinity-purified

Alternate Names for TUG Antibody - BSA Free

• Alveolar soft part sarcoma chromosomal region candidate gene 1 protein
• alveolar soft part sarcoma chromosome region, candidate 1
• Alveolar soft part sarcoma locus
• ASPLUBX domain-containing protein 9
• ASPSrenal cell carcinoma gene on chromosome 17
• RCC17renal cell carcinoma, papillary, 17
• Renal papillary cell carcinoma protein 17
• tether containing UBX domain for GLUT4
• TUG
• UBX domain protein 9
• UBXD9ASPCR1
• UBXN9FLJ45380

Background

TUG is a candidate gene for alveolar soft part sarcoma (ASPS). It has been found that this gene is fused with transcription factor TFE3 gene in ASPS and also in renal cell carcinomas. Several alternatively spliced transcript variants of this gene have been described, but their full length nature has not been determined.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: ASPSCR1