Tubulin alpha-1B Antibody (OTI3G3)

Western Blot: Tubulin alpha-1B Antibody (3G3) [NBP2-46243] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY Tubulin alpha-1B.

Immunohistochemistry: Tubulin alpha-1B Antibody (3G3) [NBP2-46243] - Analysis of Carcinoma of Human liver tissue.

Immunohistochemistry: Tubulin alpha-1B Antibody (3G3) [NBP2-46243] - Analysis of Human pancreas tissue.

Immunohistochemistry: Tubulin alpha-1B Antibody (3G3) [NBP2-46243] - Analysis of Carcinoma of Human thyroid tissue.

• Reactivity: Hu, Mu
• Applications: WB, IHC
• Clone: OTI3G3
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: 1 mg/ml

Tubulin alpha-1B Antibody (OTI3G3) Summary

• Description: Novus Biologicals Mouse Tubulin alpha-1B Antibody (OTI3G3) (NBP2-46243) is a monoclonal antibody validated for use in IHC and WB. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Full length human recombinant protein of human TUBA1B(NP_006073) produced in HEK293T cell.
• Isotype: IgG2a
• Clonality: Monoclonal
• Host: Mouse
• Gene: TUBA1B
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 1:150
  • Immunohistochemistry-Paraffin
  • Western Blot 1:4000
• Theoretical MW: 50 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.3), 1.0% BSA and 50% Glycerol
• Preservative: 0.02% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for Tubulin alpha-1B Antibody (OTI3G3)

• alpha tubulin
• Alpha-tubulin ubiquitous
• K-ALPHA-1
• tubulin alpha-1B chain
• Tubulin alpha-ubiquitous chain
• Tubulin K-alpha-1
• tubulin, alpha 1b
• tubulin, alpha, ubiquitous

Background

Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha-chain

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: TUBA1B