Tubulin alpha-1B Antibody (4D1) - Azide and BSA Free

Western Blot: Tubulin alpha-1B Antibody (4D1) [H00010376-M01] - TUBA1B monoclonal antibody (M01), clone 4D1 Analysis of TUBA1B expression in A-431.

Immunocytochemistry/Immunofluorescence: Tubulin alpha-1B Antibody (4D1) [H00010376-M01] - Analysis of monoclonal antibody to TUBA1B on A-431 cell. Antibody concentration 10 ug/ml.

Immunohistochemistry-Paraffin: Tubulin alpha-1B Antibody (4D1) [H00010376-M01] - Analysis of monoclonal antibody to TUBA1B on formalin-fixed paraffin-embedded human prostate. Antibody concentration 3 ug/ml.

Western Blot: Tubulin alpha-1B Antibody (4D1) [H00010376-M01] - Analysis of TUBA1B expression in transfected 293T cell line by TUBA1B monoclonal antibody (M01), clone 4D1.Lane 1: TUBA1B transfected lysate(50.2 KDa).Lane 2: Non-transfected lysate.

• Reactivity: Hu
• Applications: WB, ELISA, ICC/IF, IHC
• Clone: 4D1
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free

Tubulin alpha-1B Antibody (4D1) - Azide and BSA Free Summary

• Immunogen: TUBA1B (AAH08659, 1 a.a. ~ 451 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. MRECISIHVGQAGVQIGNACWELYCLKHGIQPDGQMPSDKTIGGGDDSFNTFFSETGAGKHVPRAVFVDLEPTVIDEVRTGTYRQLFHPEQLITGKEDAANNYARGHYTIGKEIIDLVLDRIRKLADQCTGLQGFLVFHSFGGGTGSGFTSLLMERLSVDYGKKSKLEFSIYPAPQVSTAVVEPYNSILTTHTTLEHSDCAFMVDNEAIYDICRRNLDIERPTYTNLNRLISQIVSSITASLRFDGALNVDLTEFQTNLVPYPRIHFPLATYAPVISAEKAYHEQLSVAEITNACFEPANQMVKCDPRHGKYMACCLLYRGDVVPKDVNAAIATIKTKRSIQFVDWCPTGFKVGINYQPPTVVPGGDLAKVQRAVCMLSNTTAIAEAWARLDHKFDLMYAKRAFVHWYVGEGMEEGEFSEAREDMAALEKDYEEVGVDSVEGEGEEEGEEY
• Marker: Microtubule Marker
• Specificity: K-ALPHA-1 - tubulin, alpha, ubiquitous
• Isotype: IgG2b Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: TUBA1B
• Purity: IgG purified

Applications/Dilutions

• Dilutions:
  • ELISA
  • Immunocytochemistry/ Immunofluorescence
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin
  • Western Blot 1:500
• Application Notes: Antibody reactive against cell lysate and recombinant protein for Western Blot. Has also been used for immunofluoresence, immunohistochemistry (paraffin), and ELISA.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: In 1x PBS, pH 7.4
• Preservative: No Preservative
• Purity: IgG purified

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for Tubulin alpha-1B Antibody (4D1) - Azide and BSA Free

• alpha tubulin
• Alpha-tubulin ubiquitous
• K-ALPHA-1
• tubulin alpha-1B chain
• Tubulin alpha-ubiquitous chain
• Tubulin K-alpha-1
• tubulin, alpha 1b
• tubulin, alpha, ubiquitous

Background

Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha-chain

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Tubulin alpha-1B Antibody (H00010376-M01)(1)

Publication using H00010376-M01

• Goers TA, Ramachran S, Aloush A et al. De novo production of K-{alpha}1 tubulin-specific antibodies: role in chronic lung allograft rejection. J Immunol;180(7):4487-94. 2008-04-01 [PMID: 18354170]

Bioinformatics

• Gene Symbol: TUBA1B
• Entrez:
  • 10376(Human)
• OMIM:
  • 602530(Human)
• Uniprot:
  • AAH08659(Human)