TTF-1 / NKX2-1 Antibody (CL14523) - Azide and BSA Free

Analysis in human thyroid gland and skeletal muscle tissues using NBP3-44302 antibody. Corresponding TTF-1 / NKX2-1 RNA-seq data are presented for the same tissues.

Staining of human liver shows no positivity in hepatocytes as expected.

Staining of human lung shows strong nuclear positivity in a subset of pneumocytes.

Staining of human skeletal muscle shows no positivity in myocytes as expected.

Staining of human thyroid gland shows strong nuclear positivity in glandular cells.

Analysis in human cell line SCLC-21H.

• Reactivity: Hu
• Applications: WB, IHC
• Clone: CL14523
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free
• Concentration: LYOPH

TTF-1 / NKX2-1 Antibody (CL14523) - Azide and BSA Free Summary

• Description: Novus Biologicals Mouse TTF-1/ NKX2-1 Antibody (CL14523) - Azide and BSA Free (NBP3-26860) is a monoclonal antibody validated for use in IHC and WB. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: This antibody was generated using a synthetic peptide of P43699, with the exact immunogen sequence remaining proprietary.
• Isotype: IgG2a
• Clonality: Monoclonal
• Host: Mouse
• Gene: NKX2-1
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry-Paraffin 1:1000 - 1:2500
  • Western Blot 1 ug/ml
• Application Notes: For IHC-Paraffin, HIER pH 6 retrieval is recommended.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: Lyophilized from a 0.2 um filtered solution in PBS with Trehalose
• Preservative: No Preservative
• Concentration: LYOPH
• Purity: Protein A purified
• Reconstitution Instructions: Centrifuge the vial of lyophilized antibody at 12,000 x g for 20 seconds. Reconstitute by adding sterile, distilled water to achieve a final antibody concentration of 1mg/ml.

Alternate Names for TTF-1 / NKX2-1 Antibody (CL14523) - Azide and BSA Free

• BCH
• benign chorea
• BHC
• Homeobox protein NK-2 homolog A
• homeobox protein Nkx-2.1
• NK2 homeobox 1
• NK-2 homolog A
• NKX2.1
• NKX2-1
• NKX2A
• NKX2ANKX2.1
• T/EBP
• Thyroid nuclear factor 1
• Thyroid transcription factor 1BHC
• TITF1
• TITF1TEBP
• TTF1
• TTF-1
• TTF1BCH
• TTF-1NK-2

Background

The thyroid transcription factor 1 (TTF-1) is a homeodomain-containing transcription factor that activates the transcriptional activity of thyroid-specific gene promoters by binding to them. Hence, TTF-1 is crucial in the maintenance of the thyroid differentiation phenotype (1). Thyroid dysgenesis (TD) is responsible for most cases of congenital hypothyroidism, a condition that affects about one in 4000 newborns. Mutations in PAX8, TTF1, or FOXE1 may account for congenital hypothyroidism in patients with either isolated TD or TD with associated malformations involving kidney, lung, forebrain, and palate (2). In addition to its presence in thyroid gland epithelium, the human TTF-1 protein was detected by immunohistochemistry in human fetal lung as early as 11 weeks of gestation, being localized in the nuclei of epithelial cells of the developing airways. After birth, TTF-1 was selectively expressed in Type II epithelial cells in the alveoli and in subsets of bronchiolar epithelial cells in the conducting regions of the lung (3).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: NKX2-1