TSLPR/CRLF2 Antibody

Western Blot: CRLF2 Antibody [NBP2-29613] - Western blot analysis of CRLF2 antibody in 1. partial recombinant CRLF2 protein 2. Human skeletal muscle lysate and 3. Human liver lysate

Immunohistochemistry-Paraffin: CRLF2 Antibody [NBP2-29613] - IHC-P analysis of CRLF2 protein in a tissue section of esophageal squamous cell carcinoma using CRLF2 antibody at a concentration of 5 ug/ml. The representative image shows a well-differentiated squamous area with distinct squamous pearl or keratin pearl formation which developed intense staining.

Immunohistochemistry-Paraffin: CRLF2 Antibody [NBP2-29613] - IHC-P analysis of CRLF2 protein in a section of normal breast tissue using CRLF2 antibody at a concentration of 5 ug/ml. The breast ductal or acinar epithelial cells showed moderate expression whereas the myoepithelial cells depicted very strong CRLF2 positivity. The interlobular stroma/connective tissue showed weak to negligible staining.

Product Discontinued

TSLPR/CRLF2 Antibody Summary

• Immunogen: Partial recombinant protein made to an internal portion of human CRLF2 protein (between residues 100-300) [NCBI AF338733_1]
• Localization: Membrane
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: CRLF2
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 5 ug/ml
  • Immunohistochemistry-Paraffin 5 ug/ml
  • Western Blot 1.0 ug/ml
• Application Notes: CRLF2 is a 371 AA long protein (42 kDa) which undergoes processing via removal of signal peptide (n-terminal 22 AA) through cleavage at AA 22, and the cleaved CRLF2 protein (349 AA) undergoes additional modifications such as glycosylation and disulfide bond formation. In our WB testing, single specific bands were observed at ~35-38 kDa position (potentially unglycosylated form) in human skeletal muscle and liver lysates. The glycosylated monomers of CRLF2 may run at ~50-60kDa position, whereas, under the non-reducing conditions, additional bands representing its dimeric/heterodimeric forms may be seen at ~120/170 kDa position (PMIDs 22915648, 21631431, 20018760, 22915648). The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• Theoretical MW: 42 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Human

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: 0.05% Sodium Azide
• Concentration: 1.0 mg/ml
• Purity: Protein A purified

Alternate Names for TSLPR/CRLF2 Antibody

• CRL2
• CRL2cytokine receptor CRL2 precusor
• CRLF2
• CRLF2Y
• CRLM-2
• Cytokine receptor-like 2
• cytokine receptor-like factor 2
• ILXR
• IL-XR
• P2RY8/CRLF2 fusion
• PCOR1
• Thymic stromal lymphopoietin protein receptor
• thymic stromal lymphopoietin receptor
• thymic stromal-derived lymphopoietin receptor
• TSLP R
• TSLP receptor
• TSLPR

Background

CRLF2 is a receptor for thymic stromal lymphopoietin (TSLP). Together with the interleukin 7 receptor (IL7R), the protein and TSLP activate STAT3, STAT5, and JAK2 pathways, which control processes such as cell proliferation and development of the hematopoietic system. Two transcript variants encoding different isoforms have been found for this gene. Recombinant human CRLF2 protein, fused to His-tag at N-terminus, was expressed in E.coli.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: CRLF2
• Entrez:
  • 64109(Human)
• Uniprot:
  • Q9HC73()