Recombinant Human TSH beta His Protein

SDS-Page: Recombinant Human TSH beta Protein [NBP2-22866]

• Reactivity: Hu
• Applications: PAGE
• Concentration: 0.25 mg/ml

Recombinant Human TSH beta His Protein Summary

• Description: A denatured recombinant protein with a N-Terminal His-tag and corresponding to the amino acids 21-138 of Human TSH beta
  
  

  Source: E.coli

  Amino Acid Sequence: MGSSHHHHHH SSGLVPRGSH MGSFCIPTEY TMHIERRECA YCLTINTTIC AGYCMTRDIN GKLFLPKYAL SQDVCTYRDF IYRTVEIPGC PLHVAPYFSY PVALSCKCGK CNTDYSDCIH EAIKTNYCTK PQKSYLVGFS V

• Source: E. coli
• Protein/Peptide Type: Recombinant Protein
• Gene: TSHB
• Purity: >85%, by SDS-PAGE

Applications/Dilutions

• Dilutions:
  • SDS-Page
• Application Notes: Denatured protein is most likely not the best option for functional studies. It is better suited for Western Blot (WB) or imaging assays.
• Theoretical MW: 15.9 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: 20mM Tris-HCl buffer (pH 8.5), 10% glycerol, 0.4M Urea
• Preservative: No Preservative
• Concentration: 0.25 mg/ml
• Purity: >85%, by SDS-PAGE

Alternate Names for Recombinant Human TSH beta His Protein

• thyroid stimulating hormone, beta
• thyroid-stimulating hormone subunit beta
• thyrotropin beta chain
• thyrotropin beta subunit
• Thyrotropin beta
• thyrotropin subunit beta
• TSH beta
• TSHB
• TSH-B
• TSH-BETA

Background

The four human glycoprotein hormones chorionic gonadotropin (CG), luteinizing hormone (LH), follicle stimulating hormone (FSH), and thyroid stimulating hormone (TSH) are dimmers consisting of alpha and beta subunits that are associated noncovalently. The alpha subunits of these hormones are identical, however, their beta chains are unique and confer biological specificity. Thyroid stimulating hormone functions in the control of thyroid structure and metabolism. TSHB is the beta subunit of thyroid stimulating hormone. Mutations in this gene result in congenital hypothyroidism. Recombinant human TSHB protein, fused to His-tag at N-terminus, was expressed in E.coli

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 3 months from date of receipt.

Bioinformatics

• Gene Symbol: TSHB