TRPM8 Antibody

• Reactivity: Hu, Mu, Rt
• Applications: ELISA
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: 0.5 mg/ml

TRPM8 Antibody Summary

• Description: Please note, this antibody is considered Innovators Grade. Innovators Grade antibodies are generally unvalidated and require additional characterization for most new species/applications. Novus has made these antibodies available through our Innovators Reward program. Complete an online review with image, detailing your positive or negative results. In return, you receive a discount voucher for 100% of the purchase price of the reviewed product. Please contact us at innovators@novusbio.com for more details.
• Immunogen: Peptide with sequence C-TSEQDKDNWNGQLK corresponding to internal region according to NP_076985.4.
• Epitope: C terminus
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: TRPM8
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Peptide ELISA Detection limit 1:8000
• Application Notes: WB: Preliminary experiments gave an approx. 28 kDa band in lysates of NIH3T3 after 1 ug/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 128 kDa band according to NP_076985.4. The 28 kDa band was successfully blocked by incubation with the immunizing peptide.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
• Preservative: 0.02% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for TRPM8 Antibody

• CMR1
• Long transient receptor potential channel 6
• LTRPC6
• LTrpC-6
• MGC2849
• short form of the TRPM8 cationic channel
• transient receptor potential cation channel, subfamily M, member 8
• Transient receptor potential p8
• transient receptor potential subfamily M member 8
• TRPM8
• TRPP8
• Trp-p8
• TRPP8transient receptor potential cation channel subfamily M member 8

Background

TRPM8 is a novel gene, trp-p8 has an expression pattern within normal human tissues that is mostly restricted to prostate epithelial cells. Trp-p8 mRNA expression is at moderate levels in normal prostate tissue and appears to be elevated in prostate cancer. Notably, trp-p8 mRNA was also expressed in a number of nonprostatic primary tumors of the breast, colon, lung and skin origin, whereas transcripts encoding trp-p8 were hardly detected or not detected in the corresponding normal human tissues.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for TRPM8 Antibody (NBP1-28499)(1)

Publication using NBP1-28499

• Dhaka A, Murray AN, Mathur J et al. TRPM8 is required for cold sensation in mice. Neuron 2007-05-03 [PMID: 17481391]

Bioinformatics

• Gene Symbol: TRPM8
• Entrez:
  • 79054(Human)
  • 171382(Mouse)
  • 171384(Rat)
• Uniprot:
  • NP_076985.4(Human)