TRPC3 Overexpression Lysate (Native)

Images

 
Western Blot: TRPC3 Overexpression Lysate (Adult Normal) [NBL1-17331] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for TRPC3.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB

Order Details

TRPC3 Overexpression Lysate (Native) Summary

Immunogen
The lysate was created in HEK293T cells, using plasmid ID RC210794 and based on accession number NM_003305. The protein contains a C-terminal DDK tag.
Specificity
Homo sapiens transient receptor potential cation channel, subfamily C, member 3 (TRPC3), transcript variant 2, mRNA.
Gene
TRPC3

Applications/Dilutions

Application Notes
This product is intended for use as a positive control in Western Blot. You will receive the lysate (100ug), and an empty vector negative control (100 ug).
Theoretical MW
97.2 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at -80C. Avoid freeze-thaw cycles.
Buffer
RIPA buffer

Lysate Details for TRPC3

Type
Overexpression
Protein State
Native

Notes

HEK293T cells in 10-cm dishes were transiently transfected with a non-lipid polymer transfection reagent specially designed and manufactured for large volume DNA transfection. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4, and then centrifuged to clarify the lysate. Protein concentration was measured by BCA protein assay kit.This product is manufactured by and sold under license from OriGene Technologies and its use is limited solely for research purposes.

Alternate Names for TRPC3 Overexpression Lysate (Native)

  • hTrp3
  • hTrp-3
  • short transient receptor potential channel 3
  • transient receptor potential cation channel, subfamily C, member 3
  • transient receptor potential channel 3
  • Transient receptor protein 3
  • TRP3
  • TRP-3
  • TrpC3

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

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Publications for TRPC3 Lysate (NBL1-17331) (0)

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Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for TRPC3 Lysate (NBL1-17331). (Showing 1 - 2 of 2 FAQ).

  1. I would like to test some of your TRPC3 antibodies in my model system (Xenopus), but they have not been proven to work in this species. Do you offer any guarantee, or beta testing program. We would be happy to send you any images we produce if the antibody works (WB, IHC, ICC). We know that TRPC1-5 are expressed in our system (Xenopus spinal cord).
    • We do offer a testing program, the Innovators Reward Program. Our Innovator’s Reward program is designed to support your innovative research with minimal financial risk to you. Should you decide to use one of our products in an application or species for which it has not been tested all you need to do is submit a full review of the antibody with an image to qualify.
  2. I am hoping you can help me concerning use of this lysate in Western Blots. How much of the lysate was loaded onto the gel used to generate the image seen on the data sheet?
    • For the image on our webpage for our TRPC3 overexpression lysate (NBL1-17331), we have loaded 10ug per lane. Please note that the blot was probed with an anti-DDK target, so your results may vary.

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Bioinformatics

Gene Symbol TRPC3