TROVE2 Antibody

Western Blot: TROVE2 Antibody [NBP2-98552] - Anti-TROVE2 rabbit polyclonal antibody at 1:500 dilution. Lane A: Jurkat Whole Cell Lysate Lane B: A431 Whole Cell Lysate Lane C: HepG2 Whole Cell Lysate Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 61 kDa. Observed band size: 61 kDa

Immunocytochemistry/Immunofluorescence: TROVE2 Antibody [NBP2-98552] - Immunofluorescence staining of TROVE2 in HeLa cells. Cells were fixed with 4% PFA, permeabilzed with 0.1% Triton X-100 in PBS, blocked with 10% serum, and incubated with rabbit anti-Human TROVE2 polyclonal antibody (dilution ratio 1:200) at 4C overnight. Then cells were stained with the Alexa Fluor(R)488-conjugated Goat Anti-rabbit IgG secondary antibody (green) and counterstained with DAPI (blue).Positive staining was localized to Cytoplasm.

Immunoprecipitation: TROVE2 Antibody [NBP2-98552] - TROVE2 was immunoprecipitated using: Lane A: 0.5 mg Jurkat Whole Cell Lysate 4 ul anti-TROVE2 rabbit polyclonal antibody and 60 ug of Immunomagnetic beads Protein A/G. Primary antibody: Anti-TROVE2 rabbit polyclonal antibody, at 1:100 dilution. Secondary antibody: Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 61 kDa. Observed band size: 61 kDa

Product Discontinued

TROVE2 Antibody Summary

• Description: This antibody can be stored at 2C to 8C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20C to -80C. Avoid repeated freeze-thaw cycles.
• Immunogen: Produced in rabbits immunized with E. coli-derived Human TROVE2 fragment.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: RO60
• Purity: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence
  • Immunoprecipitation
  • Western Blot

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.0)
• Preservative: 0.03% Proclin 300
• Purity: Antigen Affinity-purified

Alternate Names for TROVE2 Antibody

• 60 kDa ribonucleoprotein Ro
• 60 kDa Ro protein
• 60 kDa SS-A/Ro ribonucleoprotein
• Ro 60 kDa autoantigen
• RO60gastric cancer multi-drug resistance protein
• RORNP
• Sjoegren syndrome antigen A2
• Sjoegren syndrome type A antigen
• Sjogren syndrome antigen A2 (60kDa, ribonucleoprotein autoantigen SS-A/Ro)
• SS-A
• SSA2Sjogren syndrome antigen A2 (60kD, ribonucleoprotein autoantigen SS-A/Ro)
• TROVE domain family member 2
• TROVE domain family, member 2

Background

RNA-binding protein that binds to several small cytoplasmic RNA molecules known as Y RNAs. May stabilize these RNAs from degradation

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: RO60