Troponin T Type 2 (cardiac) Antibody (CL13382) - Azide and BSA Free

Analysis in human heart muscle and testis tissues using NBP3-43865 antibody. Corresponding Troponin T Type 2 (cardiac) RNA-seq data are presented for the same tissues.

Staining of human heart muscle shows strong cytoplasmic positivity in cardiomyocytes.

Staining of human liver shows no positivity in hepatocytes as expected.

Staining of human rectum shows no positivity in glandular cells as expected.

Analysis in human cell line RH-30.

Staining of human testis shows no positivity in cells in seminiferous ducts as expected.

• Reactivity: Hu
• Applications: WB, IHC
• Clone: CL13382
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free
• Concentration: LYOPH

Troponin T Type 2 (cardiac) Antibody (CL13382) - Azide and BSA Free Summary

• Description: Novus Biologicals Mouse Troponin T Type 2 (cardiac) Antibody (CL13382) - Azide and BSA Free (NBP3-24556) is a monoclonal antibody validated for use in IHC and WB. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: This antibody was generated using a synthetic peptide of P45379, with the exact immunogen sequence remaining proprietary.
• Isotype: IgG2b
• Clonality: Monoclonal
• Host: Mouse
• Gene: TNNT2
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry-Paraffin 1:10000 - 1:20000
  • Western Blot 1 ug/ml
• Application Notes: For IHC-Paraffin, we recommend using Heat-Induced Epitope Retrieval (HIER) with a pH level of 6. This optimized retrieval method ensures the best results for your experiments.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: Lyophilized from a 0.2 um filtered solution in PBS with Trehalose
• Preservative: No Preservative
• Concentration: LYOPH
• Purity: Protein A purified
• Reconstitution Instructions: Centrifuge the vial of lyophilized antibody at 12,000 x g for 20 seconds. Reconstitute by adding sterile, distilled water to achieve a final antibody concentration of 1mg/ml.

Alternate Names for Troponin T Type 2 (cardiac) Antibody (CL13382) - Azide and BSA Free

• cardiac
• CMD1D
• CMPD2
• dilated 1D (autosomal dominant)
• hypertrophic 2
• MGC3889
• RCM3
• TnTc
• troponin T type 2 (cardiac)
• troponin T, cardiac muscle

Background

Does not cross-react with skeletal muscle troponin T, cTnI or TnC.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: TNNT2