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Troponin T Type 2 (cardiac) Antibody (2F3)

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Product Details

Summary
Reactivity HuSpecies Glossary
Applications IA, S-ELISA
Clone
2F3
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
Alexa Fluor 405
NB120-10216AF405
Alexa Fluor 488
NB120-10216AF488
Alexa Fluor 647
NB120-10216AF647
Alexa Fluor 700
NB120-10216AF700
Biotin
NB120-10216B
DyLight 350
NB120-10216UV
DyLight 405
NB120-10216V
DyLight 488
NB120-10216G
DyLight 550
NB120-10216R
DyLight 650
NB120-10216C
DyLight 680
NB120-10216FR
DyLight 755
NB120-10216IR
FITC
NB120-10216F
HRP
NB120-10216H
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Troponin T Type 2 (cardiac) Antibody (2F3) Summary

Immunogen
Full length protein (Human).
Specificity
Does not cross-react with skeletal muscle troponin T, cTnI and TnC.
Isotype
IgG2b
Clonality
Monoclonal
Host
Mouse
Gene
TNNT2
Purity
Protein A purified
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Applications/Dilutions

Dilutions
  • Immunoassay
  • Sandwich ELISA 1:100-1:2000
Application Notes
It is possible to use following pairs of antibodies for sandwich immunoassay (coating - conjugate): NB120-10223 (Coating)-NB120-10216 (Conjugate)
Control
Troponin T Type 2 (cardiac) Overexpression Lysate

Reactivity Notes

Human.

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Buffer
PBS (pH 7.4)
Preservative
0.09% Sodium Azide
Purity
Protein A purified

Alternate Names for Troponin T Type 2 (cardiac) Antibody (2F3)

  • cardiac
  • CMD1D
  • CMPD2
  • dilated 1D (autosomal dominant)
  • hypertrophic 2
  • MGC3889
  • RCM3
  • TnTc
  • troponin T type 2 (cardiac)
  • troponin T, cardiac muscle

Background

Cardiac Troponin T, a 36kDa protein, is the tropomyosin binding subunit of the troponin complex, which is located on the thin filament of striated muscles and regulates muscle contraction in response to alterations in intracellular calcium ion concentration. Mutations within the protein have been associated with familial hypertrophic cardiomyopathy as well as with dilated cardiomyopathy. Transcripts for the gene undergo alternative splicing that results in many tissue specific isoforms.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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MYH7 Antibody
(940906) [Unconjugate
MAB9096
MYH7 was detected in immersion fixed paraffin-embedded sections of human heart using Mouse Anti-Human MYH7 Monoclonal Antibody (Catalog # MAB9096) at 1 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm in cardiomyocytes. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
Western blot shows lysates of human skeletal muscle tissue and human heart (atrium) tissue. PVDF membrane was probed with 0.5 µg/mL of Mouse Anti-Human MYH7 Monoclonal Antibody (Catalog # MAB9096) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for MYH7 at approximately 230 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Species: Hu
Applications: IHC, WB
MyBPC3 Antibody
AF7439
Western blot shows lysates of human heart tissue. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Human MyBPC3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7439) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for MyBPC3 at approximately 150 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.
MyBPC3 was detected in immersion fixed paraffin-embedded sections of human heart using Sheep Anti-Human MyBPC3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7439) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Sheep HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS019) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm of muscle cells. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
Species: Hu
Applications: IHC, WB
Troponin I Type 3
(cardiac) Antibody (2 ...
NBP2-35336
Western Blot: Troponin I Type 3 (cardiac) Antibody (2C9) [NBP2-35336] - Analysis of clone 2C9 in human heart at 0.5 and 1.0 ug/mL
Immunohistochemistry-Paraffin: Troponin I Type 3 (cardiac) Antibody (2C9) [NBP2-35336] - Analysis of FFPE tissue section of human heart using Troponin I antibody (clone 2C9) at 5 ug/mL concentration. The antibody specifically detected the target protein in cardiac muscle fibers in the tested sections, whereas, the nuclei as well as the intercellular collagen network did not develop any immunostaining.
Immunohistochemistry-Paraffin: Troponin I Type 3 (cardiac) Antibody (2C9) [NBP2-35336] - Formalin fixed tissue section of human breast with 5 ug/mL concentration of Troponin I Type 3 (cardiac) antibody (clone 2C9) showing no staining - Negative Control
Species: Hu, Mu
Applications: IHC, IHC-P, WB
Tropomyosin-1
Antibody
NBP1-33716
Western Blot: Tropomyosin-1 Antibody [NBP1-33716] - A. 50 ug rat muscle lysate/extract 10 % SDS-PAGETropomyosin 1 antibody dilution: 1:6000
Immunohistochemistry-Paraffin: Tropomyosin-1 Antibody [NBP1-33716] - Paraffin-embedded mouse muscle. Tropomyosin 1 antibody dilution: 1:500
Immunohistochemistry-Paraffin: Tropomyosin-1 Antibody [NBP1-33716] - Paraffin-embedded mouse vein. Tropomyosin 1 antibody dilution: 1:500.
Species: Hu, Mu, Po, Rt
Applications: ICC/IF, IHC, IHC-P, WB
Actin Alpha 1
Cardiac Muscle Antibo ...
NBP1-97725
Immunohistochemistry-Paraffin: Actin Alpha 1 Cardiac Muscle Antibody (22D3) [NBP1-97725] - Paraffin section of human cardiac muscle immunostained with 22D3 (1:500)
Immunocytochemistry/Immunofluorescence: Actin Alpha 1 Cardiac Muscle Antibody (22D3) [NBP1-97725] - Immunofluorescence staining of muscle tissue in the tail of 3 days old zebrafish embryo
Immunocytochemistry/Immunofluorescence: Actin Alpha 1 Cardiac Muscle Antibody (22D3) [NBP1-97725] - Immunofluorescence staining of developing myocardium in 1 month old zebrafish embryo
(1 Review)
(2 Publications)
Species: Bv, Gt, Hu, Mu, Po, Rb, Rt, Ze
Applications: ELISA, ICC/IF, IHC, IHC-Fr, IHC-P, WB
DMPK Antibody
(713931)
MAB6967
Western blot shows lysates of human heart tissue. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human DMPK Monoclonal Antibody (Catalog # MAB6967) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for DMPK at approximately 74 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Species: Hu
Applications: WB
Myosin Light Chain
2 Antibody
NBP1-85541
Immunohistochemistry-Paraffin: Myosin Light Chain 2 Antibody [NBP1-85541] - Analysis in human heart muscle and pancreas tissues. Corresponding Myosin Light Chain 2 RNA-seq data are presented for the same tissues.
Immunohistochemistry-Paraffin: Myosin Light Chain 2 Antibody [NBP1-85541] - Staining of human heart muscle shows moderate to strong cytoplasmic positivity in cardiomyocytes.
Immunohistochemistry-Paraffin: Myosin Light Chain 2 Antibody [NBP1-85541] - Staining of human liver shows no positivity in hepatocytes as expected.
(1 Review)
(2 Publications)
Species: Hu, Mu
Applications: IHC, IHC-Fr, IHC-P, WB
Muscleblind-like 1
Antibody (3E7)
H00004154-M02
Western Blot: Muscleblind-like 1 Antibody (3E7) [H00004154-M02] - MBNL1 monoclonal antibody (M02), clone 3E7 Analysis of MBNL1 expression in Hela S3 NE.
ELISA: Muscleblind-like 1 Antibody (3E7) [H00004154-M02] - Muscleblind-like 1 Antibody (3E7) [H00004154-M02] - Detection limit for recombinant GST tagged MBNL1 is approximately 3ng/ml as a capture antibody.
Immunocytochemistry/Immunofluorescence: Muscleblind-like 1 Antibody (3E7) [H00004154-M02] - Analysis of monoclonal antibody to MBNL1 on HeLa cell. Antibody concentration 10 ug/ml.
(2 Publications)
Species: Hu, Mu
Applications: ELISA, ICC/IF, IHC, IHC-P, WB
Myosin light chain
3 Antibody
NBP1-88068
Immunohistochemistry-Paraffin: Myosin light chain 3 Antibody [NBP1-88068] - Analysis in human heart muscle and lymph node tissues. Corresponding MYL3 RNA-seq data are presented for the same tissues.
Immunohistochemistry-Paraffin: Myosin light chain 3 Antibody [NBP1-88068] - Staining of human heart muscle shows moderate to strong cytoplasmic positivity in cardiomyocytes.
Immunohistochemistry-Paraffin: Myosin light chain 3 Antibody [NBP1-88068] - Staining of human cerebral cortex shows no positivity in neurons as expected.
Species: Hu
Applications: IHC, IHC-P
CUGBP2 Antibody
(PCRP-CELF2-1E4)
NBP3-13841
Flow Cytometry: CUGBP2 Antibody (PCRP-CELF2-1E4) [NBP3-13841] - Flow Cytometric Analysis of PFA-fixed HeLa cells. CUGBP2 antibody (PCRP-CELF2-1E4) followed by goat anti-mouse IgG-CF488 (blue); unstained cells (red).
Flow Cytometry: CUGBP2 Antibody (PCRP-CELF2-1E4) [NBP3-13841] - Flow Immunofluorescence Analysis of PFA-fixed MCF-7 cells. CUGBP2 antibody (PCRP-CELF2-1E4) followed by goat anti-mouse IgG-CF488 (green). CF640A phalloidin (red).
Flow Cytometry: CUGBP2 Antibody (PCRP-CELF2-1E4) [NBP3-13841] - Flow Cytometric Analysis of PFA-fixed U87 cells CUGBP2 antibody (PCRP-CELF2-1E4) followed by goat anti-mouse IgG-CF488 (blue); unstained cells (red).
Species: Hu
Applications: Flow, ICC/IF, PA
Troponin C
(cardiac) Antibody
NBP2-37958
Immunohistochemistry-Paraffin: Troponin C (cardiac) Antibody [NBP2-37958] - Staining of human heart muscle shows high expression.
Immunohistochemistry-Paraffin: Troponin C (cardiac) Antibody [NBP2-37958] - Staining of human skin shows low expression as expected.
Immunohistochemistry-Paraffin: Troponin C (cardiac) Antibody [NBP2-37958] - Staining in human heart muscle and skin tissues using anti-TNNC1 antibody. Corresponding TNNC1 RNA-seq data are presented for the same tissues.
Species: Hu
Applications: IHC, IHC-P
Titin Antibody
NBP1-88071
Immunohistochemistry-Paraffin: Titin Antibody [NBP1-88071] - Analysis in human skeletal muscle and liver tissues. Corresponding TTN RNA-seq data are presented for the same tissues.
Immunohistochemistry-Paraffin: Titin Antibody [NBP1-88071] - Staining of human fallopian tube shows no positivity in glandular cells as expected.
Immunohistochemistry-Paraffin: Titin Antibody [NBP1-88071] - Staining of human heart muscle shows strong cytoplasmic positivity in cardiomyocytes.
(3 Publications)
Species: Hu, Rt
Applications: ICC/IF, IHC, IHC-P
Recombinant Human
PRH2 GST (N-Term) Pro ...
H00005555-P01
SDS-Page: Recombinant Human PRH2 Protein [H00005555-P01] - 12.5% SDS-PAGE Stained with Coomassie Blue.
Species: Hu
Applications: ELISA, AP, PA, PAGE, WB
CSRP3 Antibody
NBP1-06983
Western Blot: CSRP3 Antibody [NBP1-06983] - (0.01ug/ml) staining of Human (A), Mouse (B) and Rat (C) Heart lysate (35ug protein in RIPA buffer). Detected by chemiluminescence.
Immunohistochemistry-Paraffin: CSRP3 Antibody [NBP1-06983] - Staining of paraffin embedded Human Skeletal Muscle. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
Immunohistochemistry-Paraffin: CSRP3 Antibody [NBP1-06983] - staining of paraffin embedded Human Heart. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
(2 Publications)
Species: Hu, Mu, Rt
Applications: ICC/IF, IHC, IHC-P, IP, PEP-ELISA, WB
CUGBP1/CELF1
Antibody (3B1)
NB200-316
Immunohistochemistry-Paraffin: CUGBP1/CELF1 Antibody (3B1) [NB200-316] - IHC analysis of a formalin fixed paraffin-embedded (FFPE) human spleen using 1:100 conc. of CUGBP1/CELF1 antibody on a Bond Rx autostainer (Leica Biosystems). The assay involved 30 minutes of heat induced antigen retrieval (HIER) using 10mM sodium citrate buffer (pH 9.0) and endogenous peroxidase quenching with peroxide block. The sections were incubated with primary antibody for 15 minutes and Bond Polymer Refine Detection (Leica Biosystems) with DAB was used for signal development followed by counterstaining with hematoxylin. Nuclear staining was observed in lymphocytes.
Flow Cytometry: CUGBP1 Antibody (3B1) [NB200-316] - Intracellular flow cytometric staining of 1 x 10^6 MCF-7 cells using CUGBP1 antibody (dark blue). Isotype control shown in orange. An antibody concentration of 1 ug/1x10^6 cells was used.
Simple Western: CUGBP1 Antibody (3B1) [NB200-316] - Simple Western lane view shows a specific band for CUGBP1 in 0.5 mg/ml of HeLa lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.
(12 Publications)
Species: Bv, Hu, Mu, Po, Pm, Rb, Rt
Applications: Flow, GS, ICC/IF, IHC, IHC-Fr, IHC-P, IP, Simple Western, WB
Lamin A + C
Antibody (4C4)
NBP2-25151
Immunocytochemistry/Immunofluorescence: Lamin A + C Antibody (4C4) [NBP2-25151] - Analysis of HeLa cells stained with mouse mAb to lamin A/C, NBP2-25151, dilution 1:2,000 in red, and costained with rabbit pAb to HSP60, dilution 1:5,000, in green. The blue is Hoechst staining of nuclear DNA. NBP2-25151 antibody specifically labels the nuclear lamina, while the HSP60 antibody reveals protein expressed in mitochondria.
Western Blot: Lamin A + C Antibody (4C4) [NBP2-25151] - Analysis of different cell lysates using mouse mAb to lamin A/C, NBP2-25151, dilution 1:1,000 in green: [1] protein standard (red), [2] HeLa, [3] HEK293 [4] C6, and [5] NIH-3T3 cell lysates. Two strong bands at 74 and 65kDa correspond to the lamin A and lamin C proteins respectively, detected only in the cells of human origin. NBP2-25151 antibody failed to recognize rat or mouse proteins.
Immunocytochemistry/Immunofluorescence: Lamin A + C Antibody (4C4) [NBP2-25151] - HeLa cells staing with NBP2-25151 (red), and counterstained with chicken polyclonal antibody to Vimentin NB300-223 (green) and DNA (blue). NBP2-25151 reveals strong nuclear lamina staining, while the Vimentin antibody reveals cytoplasmic intermediate filaments.
(1 Publication)
Species: Hu, Pm, Mu, Rt
Applications: ICC/IF, WB
MYH6 Antibody
(3-48) - Azide and BS ...
NB300-284
Western Blot: MYH6 Antibody (3-48) [NB300-284] - Western blot immunodetection of cardiac MyHC isoforms from left ventricle (LV) homogenates and extracts of adult euthyroid (EU), hypothyroid (HY), and hyperthyroid (TH) Lewis rats. The membranes were stained by NB300-284 antibody recognizing both MyHCalpha and MyHCbeta isoforms. Image collected and cropped by CiteAb from the following publication (http://www.hindawi.com/journals/bmri/2011/634253/), licensed under a CC-BY licence.
Western Blot: MYH6 Antibody (3-48) [NB300-284] - analysis of total cardiac myosin expression in tissue lysates of rat heart (lanes 1-5), liver (lanes 6-7), and lung (lanes 8-9). Increasing amounts of total protein were loaded as follow: lanes 1, 6 and 8: 0 ug; lane 2: 50 ug; lanes 3 , 7 and 9: 250 ug; lane 5: 500 ug. (anti-cMHC was used at 1:500; secondary antibody: Goat anti-mouse IgG Fc-HRP; 1:5000)
Immunohistochemistry-Paraffin: MYH6 Antibody (3-48) [NB300-284] - Formaldehyde-fixed paraffin-embedded (FFPE) Tissue Slides. Human heart ventricle following immunostaining with cMHC 3-48 and irrelevant NeuAc monoclonal antibody at low microscopical magnification.
(2 Reviews)
(10 Publications)
Species: Ca, Hu, Mu, Rt
Applications: ICC/IF, IHC, IHC-P, WB
LDB3 Antibody (3C8)
H00011155-M06
Western Blot: LDB3 Antibody (3C8) [H00011155-M06] - Western blot analysis of LDB3 over-expressed 293 cell line, cotransfected with LDB3 Validated Chimera RNAi or non-transfected control. Blot probed with H00011155-M06. GAPDH (36.1 kDa) used as loading control.
ELISA: LDB3 Antibody (3C8) [H00011155-M06] - Detection limit for recombinant GST tagged LDB3 is approximately 0.03ng/ml as a capture antibody.
Immunocytochemistry/Immunofluorescence: LDB3 Antibody (3C8) [H00011155-M06] - Analysis of monoclonal antibody to LDB3 on A-431 cell. Antibody concentration 10 ug/ml
(3 Publications)
Species: Hu, Mu, Rt
Applications: ELISA, ICC/IF, IP, KD, WB
CEBP Delta Antibody
NB110-85519
Western Blot: CEBP Delta Antibody [NB110-85519] - Lane 1: untreated wild-type (WT) cells. Lane 2: WT cells treated 3 hrs with LPS. Lane 3: C/EBPd-knock-out cells treated 3 hrs with LPS. 35 ug per lane. Primary: anti-C/EBPd antibody at 1:15k overnight at 4C. Blocking: buffer TBST ECL was used for detection of endogenous protein in whole cell extracts from mouse primary macrophage cells. Predicted/Observed size: 28.8 kDa, 35 kDa.
Western Blot: CEBP Delta Antibody [NB110-85519] - Lane 1: Human C/EBPbeta. Lane 2: Mouse C/EBPdelta. Lane 3: untransfected. Lane 4: Human C/EBPdelta. 35 ug per lane. Primary: Anti-C/EBPd antibody at 1:15k overnight at 4C. Blocking: buffer TBST ECL was used for detection of overexpressed protein in human and mouse C/EBP delta. Predicted/Observed size: 28.8 kDa, 35 kDa.
Western Blot: CEBP Delta Antibody [NB110-85519] - Detection of endogenous protein in whole cell extracts from mouse macrophage cells. Untreated WT cells (Lane 1), WT cells treated 3 hrs with LPS (Lane 2) and knock-out cells treated 3 hrs with LPS (Lane 3). The band at ~35 kDa, indicated by the arrowhead, corresponds to the expected molecular weight of endogenous C/EBP delta. Primary antibody at 1:15,000. ECL was used for detection.
(1 Review)
(2 Publications)
Species: Mu
Applications: ELISA, IHC, IP, KD, WB
Phospholamban
Antibody
NBP2-19807
Western Blot: Phospholamban Antibody [NBP2-19807] - Various tissue extracts (50 ug) were separated by 15% SDS-PAGE, and the membrane was blotted with Phospholamban antibody diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.
Immunohistochemistry-Paraffin: Phospholamban Antibody [NBP2-19807] - Human colon carcinoma, using phospholamban antibody at 1:250 dilution.Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.
(3 Publications)
Species: Hu, Mu, Po, Rt
Applications: ICC/IF, IHC, IHC-P, WB

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Control Lysate(s)

Troponin T Type 2 (cardiac) Overexpression Lysate

Secondary Antibodies

 

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Bioinformatics

Gene Symbol TNNT2
Entrez
Uniprot