Troponin I Type 1 (slow skeletal) Antibody (12F10) - BSA Free

• Reactivity: Hu
• Applications: WB, ELISA, AP
• Clone: 12F10
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: BSA Free

Troponin I Type 1 (slow skeletal) Antibody (12F10) - BSA Free Summary

• Immunogen: Hybridoma clone has been derived from hybridization of Sp2/0 myeloma cells with spleen cells of Balb/c mice immunized with Human skTnI.
• Specificity: Troponin I from human skeletal muscle. Cross-reaction of this with cardiac troponin I is less then 0.05%.
• Isotype: IgG2b
• Clonality: Monoclonal
• Host: Mouse
• Gene: TNNI1
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Immunoaffinity Purification
  • Immunoassay
  • Sandwich ELISA
  • Western Blot
• Application Notes: Recommended pair for sandwich immunoassay (coating - conjugate): NB200-431 (Coating) - NB200-432 (conjugate)
• Control:

  Troponin T type 1 (slow skeletal) Overexpression Lysate

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: PBS (pH 7.4)
• Preservative: 0.09% Sodium Azide
• Purity: Protein A purified

Alternate Names for Troponin I Type 1 (slow skeletal) Antibody (12F10) - BSA Free

• DKFZp451O223
• slow-twitch isoform
• SSTNI
• TNN1
• troponin I type 1 (skeletal, slow)
• troponin I, skeletal, slow
• troponin I, slow skeletal muscle

Background

Troponin I is part of a heteromeric complex playing an important role in the regulation of skeletal and cardiac muscle contraction. It consists of three subunits, troponin I (TnI), troponin T (TnT) and troponin C (TnC). Each subunit is responsible for part of troponin complex function. TnI inhibits ATPase activity of acto myosin and TnT and TnI are present in cardiac muscles in different forms than in skeletal muscles. Only one tissue specific isoform of TnI is described for cardiac muscle tissue (cTnI) and this is expressed only in myocardium. This antibody reacts with subunit I of the troponin complex in human myocardium, where it is the only troponin I isotype present. It does not cross-react with troponin I of skeletal muscle cells.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: TNNI1
• Entrez:
  • 7135(Rat)
• Uniprot:
  • P19237(Human)