Troponin T type 1 (slow skeletal) Overexpression Lysate (Native)

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Western Blot: Troponin T type 1 (slow skeletal) Overexpression Lysate (Adult Normal) [NBL1-17179] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for Troponin T type ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB

Order Details

Troponin T type 1 (slow skeletal) Overexpression Lysate (Native) Summary

Immunogen
The lysate was created in HEK293T cells, using plasmid ID RC221318 and based on accession number NM_003283. The protein contains a C-terminal DDK tag.
Specificity
Homo sapiens Troponin T type 1 (slow skeletal), mRNA.
Gene
TNNT1

Applications/Dilutions

Application Notes
This product is intended for use as a positive control in Western Blot. You will receive the lysate (100ug), and an empty vector negative control (100 ug).
Theoretical MW
32.8 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at -80C. Avoid freeze-thaw cycles.
Buffer
RIPA buffer

Lysate Details for Troponin T type 1 (slow skeletal)

Type
Overexpression
Protein State
Native

Notes

HEK293T cells in 10-cm dishes were transiently transfected with a non-lipid polymer transfection reagent specially designed and manufactured for large volume DNA transfection. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4, and then centrifuged to clarify the lysate. Protein concentration was measured by BCA protein assay kit.This product is manufactured by and sold under license from OriGene Technologies and its use is limited solely for research purposes.

Alternate Names for Troponin T type 1 (slow skeletal) Overexpression Lysate (Native)

  • ANMtroponin T, slow skeletal muscle
  • FLJ98147
  • MGC104241
  • Slow skeletal muscle troponin T
  • STNT
  • TnTs
  • TNTtroponin T1, skeletal, slow
  • troponin T type 1 (skeletal, slow)
  • troponin-T1, skeletal, slow

Background

This gene encodes a protein that is a subunit of troponin, which is a regulatory complex located on the thin filament of the sarcomere. This complex regulates striated muscle contraction in response to fluctuations in intracellular calcium concentration. This complex is composed of three subunits: troponin C, which binds calcium, troponin T, which binds tropomyosin, and troponin I, which is an inhibitory subunit. This protein is the slow skeletal troponin T subunit. Mutations in this gene cause nemaline myopathy type 5, also known as Amish nemaline myopathy, a neuromuscular disorder characterized by muscle weakness and rod-shaped, or nemaline, inclusions in skeletal muscle fibers which affects infants, resulting in death due to respiratory insufficiency, usually in the second year. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq]. Transcript Variant: This variant (1) represents the longest transcript and encodes the longest isoform (a). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

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Publications for Troponin T type 1 (slow skeletal) Lysate (NBL1-17179) (0)

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Bioinformatics

Gene Symbol TNNT1