TRMU Antibody (OTI2A4) - Azide and BSA Free

Images

 
Western Blot: TRMU Antibody (OTI2A4) - Azide and BSA Free [NBP2-74629] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY TRMT1.
Immunocytochemistry/ Immunofluorescence: TRMU Antibody (OTI2A4) - Azide and BSA Free [NBP2-74629] - Analysis of COS7 cells transiently transfected by pCMV6-ENTRY TRMT1.
Western Blot: TRMU Antibody (OTI2A4) - Azide and BSA Free [NBP2-74629] - Analysis of extracts (35ug) from 9 different cell lines (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: ...read more

Product Details

Summary
Reactivity Hu, Mu, Rt, Ca, PmSpecies Glossary
Applications WB, ICC/IF
Clone
OTI2A4
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Format
Azide and BSA Free
Concentration
LYOPH

Order Details

TRMU Antibody (OTI2A4) - Azide and BSA Free Summary

Immunogen
Full length human recombinant protein of human TRMU(NP_060476) produced in HEK293T cell.
Isotype
IgG2a
Clonality
Monoclonal
Host
Mouse
Gene
TRMU
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunocytochemistry/Immunofluorescence 1:100
  • Western Blot 1:200-4000
Theoretical MW
47.6 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
Lyophilized from PBS (pH 7.3) with 8% Trehalose
Preservative
No Preservative
Concentration
LYOPH
Purity
Immunogen affinity purified
Reconstitution Instructions
Reconstitute with PBS (pH 7.3). To use this carrier-free antibody for conjugation experiments, another round of desalting is highly recommended.

Alternate Names for TRMU Antibody (OTI2A4) - Azide and BSA Free

  • EC 2.8.1
  • EC 2.8.1.-
  • FLJ10140
  • mitochondrial 5-methylaminomethyl-2-thiouridylate-methyltransferase
  • mitochondrial tRNA-specific 2-thiouridylase 1
  • MTO2 homolog
  • MTO2MGC99627
  • MTU1
  • TRMT
  • TRMT1
  • tRNA (5-methylaminomethyl-2-thiouridylate)-methyltransferase
  • tRNA 5-methylaminomethyl-2-thiouridylate methyltransferase
  • TRNT1

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Bioinformatics

Gene Symbol TRMU