MTO1 Antibody

Images

 
Western Blot: MTO1 Antibody [NBP1-54767] - Detection of MTO1 in HeLa whole cell lysate enriched for mitochondria via digitonin treatment prior to cell pelleting (Hm lane) and human fibroblast whole cell lysate (Fi ...read more
Western Blot: MTO1 Antibody [NBP1-54767] - Titration: 0.2-1 ug/ml, Positive Control: Human Muscle.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
0.5 mg/ml

Order Details

MTO1 Antibody Summary

Description
The addition of 50% glycerol is optional for those storing this antibody at -20C and not aliquoting smaller units. However, please note that glycerol may interrupt some downstream antibody applications and should be added with caution.
Immunogen
Synthetic peptides corresponding to MTO1(mitochondrial translation optimization 1 homolog (S. cerevisiae)) The peptide sequence was selected from the N terminal of MTO1 (NP_001116698). Peptide sequence STVYAESVILTTGTFLRGMIVIGLETHPAGRLGDQPSIGLAQTLEKLGFV. The peptide sequence for this immunogen was taken from within the described region.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
MTO1
Purity
Affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1.0 ug/ml
Theoretical MW
81 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Reviewed Applications
Read 1 Review rated 4
using
NBP1-54767 in the following application:

Publications
Read Publication using NBP1-54767.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS, 2% Sucrose
Preservative
0.09% Sodium Azide
Concentration
0.5 mg/ml
Purity
Affinity purified

Alternate Names for MTO1 Antibody

  • EC 1.1.1.40
  • EC 6.3.5
  • homolog of yeast Mto1
  • mitochondrial MTO1-3
  • mitochondrial translation optimization 1 homolog (S. cerevisiae)
  • protein MTO1 homolog, mitochondrial

Background

MTO1 is a mitochondrial protein thought to be involved in mitochondrial tRNA modification. It may also play a role in the expression of the non-syndromic and aminoglycoside-induced deafness phenotypes associated with a specific mutation in the mitochondrial 12S rRNA gene.This gene encodes a mitochondrial protein thought to be involved in mitochondrial tRNA modification. The encoded protein may also play a role in the expression of the non-syndromic and aminoglycoside-induced deafness phenotypes associated with a specific mutation in the mitochondrial 12S rRNA gene. Multiple transcript variants encoding different isoforms have been found for this gene.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customers Who Viewed This Item Also Viewed...

NBP2-46016
Species: Ca, Hu, Pm, Mu, Rt
Applications: ICC/IF, IF, WB
NBP1-85500
Species: Hu, Mu
Applications: ICC/IF, IHC, IHC-P, Simple Western, WB
NBP1-91974
Species: Hu, Mu, Rt
Applications: ICC/IF, IHC, IHC-P, WB
NBP1-87797
Species: Hu, Mu, Rt
Applications: IHC, IHC-P, Simple Western, WB
NBP2-41304
Species: Hu, Po, Rt
Applications: ELISA, ICC/IF, IHC, IHC-P, WB
NBP1-84621
Species: Hu, Mu, Rt
Applications: ICC/IF, IHC, IHC-P, WB
G-125-C
Species: Hu
Applications:
H00728695-B01P
Species: Hu
Applications: ICC/IF, IP, WB
NBP1-88927
Species: Hu, Mu
Applications: ICC/IF, IHC, IHC-P, WB
H00010312-M01
Species: Hu, Mu
Applications: ELISA, IHC, IHC-Fr, WB
NBP1-43435
Species: Hu, Mu
Applications: Flow, WB
NB100-689
Species: Hu, Rt
Applications: IHC, IHC-Fr, IHC-P, Simple Western, WB
H00027349-M01
Species: Hu
Applications: ELISA, IHC, IHC-P, S-ELISA, WB
NBP2-94912
Species: Hu, Mu, Rt
Applications: WB
MAB4470
Species: All-Multi
Applications: ICC, IHC, WB
H00002707-M01
Species: Hu
Applications: ELISA, ICC/IF, S-ELISA, WB
NBP1-85471
Species: Hu
Applications: ICC/IF, IHC, IHC-P
NBP2-81948
Species: Hu, Mu, Rt
Applications: ELISA, ICC/IF, WB

Publications for MTO1 Antibody (NBP1-54767)(1)


Showing Publication 1 - 1 of 1.
Publication using NBP1-54767 Applications Species
Krull,M. Mol. Biol. Evol. 22 (8), 1702-1711. 2005-01-01 [PMID: 15901843]

Review for MTO1 Antibody (NBP1-54767) (1) 41

Average Rating: 4
(Based on 1 review)
We have 1 review tested in 1 species: Human.

Reviews using NBP1-54767:
Filter by Applications
WB
(1)
All Applications
Filter by Species
Human
(1)
All Species
Images Ratings Applications Species Date Details
Western Blot MTO1 NBP1-54767
Enlarge
4
reviewed by:
Maria Holmström
WB Human 08/23/2013
View

Summary

ApplicationWestern Blot
Sample TestedHeLA whole cell lysate and lysate enriched for mitochondria by digitonin treatment; human fibroblast whole cell lysate
SpeciesHuman
LotQC23680

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for MTO1 Antibody (NBP1-54767) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.
mFlour Violet Conjugated Antibodies

Customers Who Bought This Also Bought

Contact Information

Product PDFs

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Recent Reviews

4
5
0
4
1
3
0
2
0
1
0

Maria Holmström
08/23/2013
Application: WB
Species: Human

Bioinformatics

Gene Symbol MTO1
Entrez
Uniprot