TRIM9 Antibody (1F12) - Azide and BSA Free

Western Blot: TRIM9 Antibody (1F12) [H00114088-M01] - Analysis of TRIM9 expression in transfected 293T cell line by TRIM9 monoclonal antibody (M01), clone 1F12.Lane 1: TRIM9 transfected lysate(61.3 KDa).Lane 2: Non-transfected lysate.

• Reactivity: Hu, Mu
• Applications: WB, ELISA, IP
• Clone: 1F12
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free

TRIM9 Antibody (1F12) - Azide and BSA Free Summary

• Immunogen: TRIM9 (NP_055978.4, 1 a.a. ~ 109 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. MEEMEEELKCPVCGSFYREPIILPCSHNLCQACARNILVQTPESESPQSHRAAGSGVSDYDYLDLDKMSLYSEADSGYGSYGGFASAPTTPCQKSPNGVRVFPPAMPPP
• Specificity: TRIM9 - tripartite motif-containing 9
• Isotype: IgG2b Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: TRIM9
• Purity: IgG purified

Applications/Dilutions

• Dilutions:
  • ELISA 1:100-1:2000
  • Immunoprecipitation
  • Western Blot 1:500
• Application Notes: Antibody reactive against recombinant protein on ELISA. It has also been used for Western Blot.

Reactivity Notes

Other species not tested.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: In 1x PBS, pH 7.4
• Preservative: No Preservative
• Purity: IgG purified

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for TRIM9 Antibody (1F12) - Azide and BSA Free

• EC 6.3.2.-
• KIAA0282homolog of rat RING finger Spring
• RNF91RING finger protein 91
• SPRINGE3 ubiquitin-protein ligase TRIM9
• TRIM9
• tripartite motif containing 9
• tripartite motif-containing 9
• Tripartite motif-containing protein 9

Background

The protein encoded by this gene is a member of the tripartite motif (TRIM) family. The TRIM motif includes three zinc-binding domains, a RING, a B-box type 1 and a B-box type 2, and a coiled-coil region. The protein localizes to cytoplasmic bodies. Its function has not been identified. Alternate splicing of this gene generates two transcript variants encoding different isoforms.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for TRIM9 Antibody (H00114088-M01)(3)

Publications using H00114088-M01

• Nicholas B, Laura M, Shalini M et al. A pair of E3 ubiquitin ligases compete to regulate filopodial dynamics and axon guidance. J Cell Biol. 2020-01-06 [PMID: 31820781]
• Shi M, Cho H, Inn KS et al. Negative regulation of NF-kB activity by brain-specific TRIpartite Motif protein 9. Nat Commun. 2014-09-05 [PMID: 25190485]
• Winkle CC, McClain LM, Valtschanoff JG et al. A novel Netrin-1-sensitive mechanism promotes local SNARE-mediated exocytosis during axon branching. J Cell Biol. 2014-04-28 [PMID: 24778312]

Bioinformatics

• Gene Symbol: TRIM9
• Entrez:
  • 114088(Human)
• OMIM:
  • 606555(Human)
• Uniprot:
  • NP_055978.4(Human)