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TRIM5 Overexpression Lysate

Images

 
Western Blot: TRIM5 Overexpression Lysate (Adult Normal) [NBL1-17303] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for TRIM5.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB

Order Details

TRIM5 Overexpression Lysate Summary

Description
Transient overexpression lysate of tripartite motif-containing 5 (TRIM5), transcript variant gamma The lysate was created in HEK293T cells, using Plasmid ID RC205696 and based on accession number NM_033092. The protein contains a C-MYC/DDK Tag.
Gene
TRIM5

Applications/Dilutions

Dilutions
  • Western Blot
Application Notes
This product is intended for use as a positive control in Western Blot.

You will receive 1 vial of lysate (100ug), 1 vial of empty vector negative control (100ug), and 1 vial of 2xSDS sample buffer (250ul).
Theoretical MW
39.9 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at -80C. Avoid freeze-thaw cycles.
Buffer
RIPA buffer

Lysate Details for Array

Type
Overexpression

Notes

HEK293T cells in 10-cm dishes were transiently transfected with a non-lipid polymer transfection reagent specially designed and manufactured for large volume DNA transfection. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4, and then centrifuged to clarify the lysate. Protein concentration was measured by BCA protein assay kit.This product is manufactured by and sold under license from OriGene Technologies and its use is limited solely for research purposes.

Alternate Names for TRIM5 Overexpression Lysate

  • EC 6.3.2
  • EC 6.3.2.-
  • RING finger protein 88
  • RNF88
  • RNF88tripartite motif protein TRIM
  • TRIM5
  • TRIM5alpha
  • tripartite motif containing 5
  • tripartite motif protein TRIM5
  • tripartite motif-containing 5
  • tripartite motif-containing protein 5

Background

The protein encoded by this gene is a member of the tripartite motif (TRIM) family. The TRIM motif includes three zinc-binding domains, a RING, a B-box type 1 and a B-box type 2, and a coiled-coil region. The protein localizes to cytoplasmic bodies. Its function has not been identified. Five alternatively spliced transcript variants for this gene have been described. However, the full length nature of two of the variants has not been determined. [provided by RefSeq]. Transcript Variant: This variant (gamma) undergoes alternate splicing within the coding region, resulting in a frameshift and use of an upstream termination codon, compared to variant alpha. Its encoded isoform has a distinct C-terminus, compared to the other isoforms. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

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Publications for TRIM5 Lysate (NBL1-17303) (0)

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Bioinformatics

Gene Symbol TRIM5