TRIM5 Antibody [Alexa Fluor® 647]

• Applications: WB
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Alexa Fluor 647

TRIM5 Antibody [Alexa Fluor® 647] Summary

• Immunogen: E. coli-derived recombinant human TRIM5
  Ala2-Gln248
  Accession # Q9C035
• Specificity: Detects human TRIM5 in direct ELISAs and Western blots. In direct ELISAs, less than 5% cross-reactivity with recombinant mouse TRIM5 is observed.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Purity Statement: Antigen Affinity-purified

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied
• Buffer: Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Background

TRIM5 belongs to the large family of TRIM proteins, which are also named RBCC proteins. These proteins contain a RING domain, one or two B-boxes (CHC3H2 zinc finger motifs), and the coiled-coil domain. Different TRIM proteins contain C-terminals that vary in length and composition. They also have different functions and cellular localization. TRIM5 possesses a B30.2 (also known as RFP-like or PRY-SPRY) C-terminal domain and exists as a homotrimer. TRIM5 is a single protein RING finger E3 ubiquitin ligase and has been shown to have antiretroviral activities. Five splice variants that have identical amino-terminal residues 1‑248, but differ in their C-terminal regions have been reported. Human TRIM5 shares 49% amino acid sequence homology with mouse TRIM5.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.