TRIM5 alpha Antibody (3B11H2)

Western Blot: TRIM5 alpha Antibody (3B11H2) [NB100-2192] - Analysis using Trim5a mouse monoclonal antiobdy against truncated Trim5arecombinant protein.

Immunohistochemistry: TRIM5 alpha Antibody (3B11H2) [NB100-2192] - Analysis of human metastatic adenocarcinoma(A) and stomach adenocarcinoma (B), showing cytoplasmic localization using Trim5a mouse mAb with AEC staining (A) and DAB staining(B).

Western Blot: TRIM5 alpha Antibody (3B11H2) [NB100-2192] - Analysis using Trim5a mouse mAb against human breast carcinoma tissue lysate.

Product Discontinued

TRIM5 alpha Antibody (3B11H2) Summary

• Immunogen: Purified recombinant fragment of human trim5 alpha expressed in E. Coli.
• Localization: Cytoplasmic bodies
• Specificity: TRIM5 alpha (3B11H2)
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: TRIM5
• Purity: Unpurified

Applications/Dilutions

• Dilutions:
  • ELISA 1:10000
  • Immunohistochemistry 1:200 - 1:1000
  • Immunohistochemistry-Paraffin 1:100 - 1:400
  • Western Blot 1:1000 - 1:5000

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: Ascites
• Preservative: 0.03% Sodium Azide
• Purity: Unpurified

Alternate Names for TRIM5 alpha Antibody (3B11H2)

• EC 6.3.2
• EC 6.3.2.-
• RING finger protein 88
• RNF88tripartite motif protein TRIM
• TRIM5alpha
• tripartite motif containing 5
• tripartite motif protein TRIM5
• tripartite motif-containing 5
• tripartite motif-containing protein 5

Background

TRIM5-alpha is a protein that is found in the cells of many mammals and fends of various retrovirus infections. It protects monkeys from infection with HIV-1, and humans from infection with some other viruses. If a retrovirus has entered a cell, it needs to shed its capsid in order to reversely transcribe its genes, so that they can be expressed by the host cell. It is believed that TRIM5 alpha, which is present in the cytoplasm, somehow recognizes the capsid and blocks its shedding, thereby stopping the virus in its tracks. It thus represents an intracellular defense completely separate from the rest of the body's immune system.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: TRIM5
• Entrez:
  • 85363(Human)