TRIM4 Antibody

Western Blot: TRIM4 Antibody [NB100-1225] - Detection of TRIM4 in HepG2 lysate (RIPA buffer, 30 ug total protein per lane) using this antibody at 1 ug/ml.

• Reactivity: Hu
• Applications: WB, ELISA
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: 0.5 mg/ml

TRIM4 Antibody Summary

• Description: Novus Biologicals Goat TRIM4 Antibody (NB100-1225) is a polyclonal antibody validated for use in WB and ELISA. Anti-TRIM4 Antibody: Cited in 1 publication. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Peptide with sequence C-ASLVIPPVTDRK corresponding to C-Terminus according to NP_148977.1, NP_149082.1.
• Specificity: This antibody is expected to recognise both reported human isoforms (as represented by NP_148977 and NP_149082).
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: TRIM4
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Peptide ELISA Detection limit 1:32000
  • Western Blot 1 ug/ml
• Application Notes: WB: Approx. 55-60 kDa band observed in HepG2 whole lysate (predicted MW of 58 kDa band and 55 kDa band according to NP_148977 and NP_149082).

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
• Preservative: 0.02% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for TRIM4 Antibody

• RING finger protein 87
• RNF87tripartite motif protein 4
• tripartite motif containing 4
• tripartite motif-containing 4
• tripartite motif-containing protein 4

Background

The protein encoded by this gene is a member of the tripartite motif (TRIM) family. The TRIM motif includes three zinc-binding domains, a RING, a B-box type 1 and a B-box type 2, and a coiled-coil region. The protein localizes to cytoplasmic bodies. Its function has not been identified. Alternative splicing of this gene generates two transcript variants.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for TRIM4 Antibody (NB100-1225)(1)

Publication using NB100-1225

• Reymond A, Meroni G, Fantozzi A et al. The tripartite motif family identifies cell compartments. EMBO J 2001-05-01 [PMID: 11331580]

Bioinformatics

• Gene Symbol: TRIM4
• Entrez:
  • 89122(Human)
• Uniprot:
  • NP_148977(Human)