TRIM21/SSA1 Antibody (672714) [Alexa Fluor® 405]

• Reactivity: Hu
• Applications: WB, IP
• Clone: 672714
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Alexa Fluor 405

TRIM21/SSA1 Antibody (672714) [Alexa Fluor® 405] Summary

• Immunogen: E. coli-derived recombinant human TRIM21
  Arg195-Pro293
  Accession # P19474
• Specificity: Detects human TRIM21 in direct ELISAs and Western blots. In direct ELISAs, no cross-reactivity with recombinant human (rh) TRIM5 or rhTRIM32 is observed.
• Isotype: IgG2a
• Clonality: Monoclonal
• Host: Mouse
• Purity Statement: Protein A or G purified

Applications/Dilutions

• Dilutions:
  • Immunoprecipitation
  • Western Blot

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied
• Buffer: Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for TRIM21/SSA1 Antibody (672714) [Alexa Fluor® 405]

• 52 kDa ribonucleoprotein autoantigen Ro/SS-A
• 52 kDa Ro/SSA
• E3 ubiquitin-protein ligase TRIM21
• EC 6.3.2.-
• rhTRIM21
• RNF81
• RNF81RING finger protein 81
• ro(SS-A)
• Ro52
• RO52SS-A
• Sicca syndrome antigen A
• Sjoegren syndrome type A antigen
• Sjogren syndrome antigen A1 (52kDa, ribonucleoprotein autoantigen SS-A/Ro)
• SSA1
• SSA1SSA
• TRIM21
• tripartite motif containing 21,52 kDa Ro protein
• tripartite motif-containing 21
• Tripartite motif-containing protein 21

Background

TRIM21 (Tripartite motif-containing protein 21; also Ro(SS-A), 52 kDa Ro Protein/Ro52, and RING finger protein 81) is a 52-56 kDa member of the RING finger-B-box-coiled-coil family of proteins. It is an E3 ligase that is found in both nucleus and cytoplasm, where it is often associated with microtubules. TRIM21 ubiquitinates select proteins. In B cells, it targets the Fc fragment of misfolded IgG, providing QC on its production. In macrophages, it acts in a nondegradative manner on IRF8, promoting innate immunity. Human TRIM21 is 475 amino acids (aa) in length. It contains one E3 ligase RING finger domain (aa 16-55), a B-Box type zinc-finger region (aa 92-123), a coiled-coil region (aa 128-238) and a C-terminal SPRY/B30.2 Ig binding domain (aa 268-465). TRIM21 is reported to form trimers. Over aa 195‑293, human TRIM21 exhibits 72% aa identity with mouse TRIM21.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.