TRIM16 Antibody (5F4) - Azide and BSA Free

Western Blot: TRIM16 Antibody (5F4) [H00010626-M01] - Analysis of TRIM16 expression in transfected 293T cell line by TRIM16 monoclonal antibody (M01), clone 5F4.Lane 1: TRIM16 transfected lysate(64 KDa).Lane 2: Non-transfected lysate.

Western Blot: TRIM16 Antibody (5F4) [H00010626-M01] - Analysis of TRIM16 over-expressed 293 cell line, cotransfected with TRIM16 Validated Chimera RNAi ( Cat # H00010626-R01V ) (Lane 2) or non-transfected control (Lane 1). Blot probed with TRIM16 monoclonal antibody (M01), clone 5F4 (Cat # H00010626-M01 ). GAPDH ( 36.1 kDa ) used as specificity and loading control.

Immunoprecipitation: TRIM16 Antibody (5F4) [H00010626-M01] - Analysis of TRIM16 transfected lysate using anti-TRIM16 monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with TRIM16 MaxPab rabbit polyclonal antibody.

Sandwich ELISA: TRIM16 Antibody (5F4) [H00010626-M01] - Detection limit for recombinant GST tagged TRIM16 is approximately 0.03 ng/mL as a capture antibody.

Simple Western: TRIM16 Antibody (5F4) [H00010626-M01] - This antibody (1:25 dilution) was used to probe THP-1 macrophage lysate (concentrations shown). Simple Western image submitted by a verified customer review.

• Reactivity: Hu
• Applications: WB, Simple Western, ELISA, IP, KD
• Clone: 5F4
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free

TRIM16 Antibody (5F4) - Azide and BSA Free Summary

• Immunogen: TRIM16 (NP_006461, 165 a.a. ~ 273 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. LDAARRDKEAELQCTQLDLERKLKLNENAISRLQANQKSVLVSVSEVKAVAEMQFGELLAAVRKAQANVMLFLEEKEQAALSQANGIKAHLEYRSAEMEKSKQELERMA
• Specificity: TRIM16 - tripartite motif-containing 16
• Isotype: IgG2a Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: TRIM16
• Purity: IgG purified

Applications/Dilutions

• Dilutions:
  • ELISA
  • Immunoprecipitation
  • Knockdown Validated
  • Sandwich ELISA
  • Simple Western
  • Western Blot 1:500
• Application Notes: Antibody reactive against recombinant protein for Western blot. It has also been used for RNAi validation and ELISA. This TRIM16 Antibody (5F4) is validated for Simple Western from a verified customer review.
  See Simple Western Antibody Database for Simple Western validation: Tested in THP-1 macrophage lysate 0.2 mg/mL, 0.6 mg/mL and 1.2 mg/mL, separated by Size, antibody dilution of 1:25

Reactivity Notes

Human. Other species not tested.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: In 1x PBS, pH 7.4
• Preservative: No Preservative
• Purity: IgG purified

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for TRIM16 Antibody (5F4) - Azide and BSA Free

• DKFZp434O1826
• EBBPtripartite motif-containing 16
• Estrogen-responsive B box protein
• tripartite motif containing 16
• tripartite motif-containing protein 16

Background

This gene was identified as an estrogen and anti-estrogen regulated gene in epithelial cells stably expressing estrogen receptor. The protein encoded by this gene contains two B box domains and a coiled-coiled region that are characteristic of the B box zinc finger protein family. The proteins of this family have been reported to be involved in a variety of biological processes including cell growth, differentiation and pathogenesis. Expression of this gene was detected in most tissues. Its function, however, has not yet been determined.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Review for TRIM16 Antibody (H00010626-M01) (1)

Average Rating: 3

(Based on 1 review)

We have 1 review tested in 1 species: Human.

reviewed by: Verified Customer Simple Western Human 12/05/2019

Summary

• Application: Simple Western
• Sample Tested: THP-1 macrophage lysate
• Species: Human
• Lot: I9191-5F4

Bioinformatics

• Gene Symbol: TRIM16
• Entrez:
  • 10626(Human)
• OMIM:
  • 609505(Human)
• Uniprot:
  • NP_006461(Human)