TREX1 Antibody (JG35-71)

Western Blot: TREX1 Antibody (JG35-71) [NBP2-76875] - Western blot analysis of TREX1 on different cell lysates using anti-TREX1 at 1/500 dilution.Positive control:Line 1: A431 Line 2: SK-Br-3

Immunocytochemistry/Immunofluorescence: TREX1 Antibody (JG35-71) [NBP2-76875] - ICC staining TREX1 in SiHa cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Immunohistochemistry: TREX1 Antibody (JG35-71) [NBP2-76875] - Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue using anti-TREX1 antibody. Counter stained with hematoxylin.

Immunocytochemistry/Immunofluorescence: TREX1 Antibody (JG35-71) [NBP2-76875] - ICC staining TREX1 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Immunocytochemistry/Immunofluorescence: TREX1 Antibody (JG35-71) [NBP2-76875] - ICC staining TREX1 in LOVO cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Immunohistochemistry: TREX1 Antibody (JG35-71) [NBP2-76875] - Immunohistochemical analysis of paraffin-embedded human colon tissue using anti-TREX1 antibody. Counter stained with hematoxylin.

Product Discontinued

TREX1 Antibody (JG35-71) Summary

• Additional Information: Recombinant Monoclonal Antibody.
• Immunogen: Recombinant protein within Human TREX1 aa 110-314 / 314. (SwissProt: Q9NSU2 Human)
• Localization: Cytoplasm, Endoplasmic reticulum, Membrane, Nucleus.
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Gene: TREX1
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:50-1:200
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 1:50-1:200
  • Western Blot 1:500-1:2000
• Theoretical MW: 33 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: TBS (pH7.4), 0.05% BSA, 40% Glycerol
• Preservative: 0.05% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Protein A purified

Alternate Names for TREX1 Antibody (JG35-71)

• 3'-5' exonuclease TREX1
• AGS1
• Aicardi-Goutieres syndrome 1
• CRV
• DKFZp434J0310
• DNase III
• DRN3
• EC 3.1.11.2
• HERNS
• three prime repair exonuclease 1,3' repair exonuclease 1

Background

TREX1 encodes the major 3'->5' DNA exonuclease in human cells. The protein is a non-processive exonuclease that may serve a proofreading function for a human DNA polymerase. It is also a component of the SET complex, and acts to rapidly degrade 3' ends of nicked DNA during granzyme A-mediated cell death. Mutations in this gene result in Aicardi-Goutieres syndrome, chilblain lupus, and Cree encephalitis. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: TREX1