RASD2 Antibody (OTI1F7)

Western Blot: RASD2 Antibody (1F7) [NBP2-03331] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY RASD2 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-RASD2.

Immunocytochemistry/Immunofluorescence: RASD2 Antibody (1F7) [NBP2-03331] Staining of COS7 cells transiently transfected by pCMV6-ENTRY RASD2.

Immunohistochemistry-Paraffin: RASD2 Antibody (1F7) [NBP2-03331] - Staining of paraffin-embedded Carcinoma of Human prostate tissue using anti-RASD2 mouse monoclonal antibody.

Flow Cytometry: RASD2 Antibody (1F7) [NBP2-03331] - HEK293T cells transfected with either overexpression plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-RASD2 antibody, and then analyzed by flow cytometry.

Immunohistochemistry-Paraffin: RASD2 Antibody (1F7) [NBP2-03331] - Staining of paraffin-embedded Carcinoma of Human bladder tissue using anti-RASD2 mouse monoclonal antibody.

• Reactivity: Hu, Mu, Rt
• Applications: WB, Flow, ICC/IF, IHC
• Clone: OTI1F7
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: 1 mg/ml

RASD2 Antibody (OTI1F7) Summary

• Immunogen: Full length human recombinant protein of human RASD2 (NP_055125) produced in HEK293T cell.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: RASD2
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 1:100
  • Immunocytochemistry/Immunofluorescence 1:100
  • Immunohistochemistry 1:150
  • Immunohistochemistry-Paraffin 1:150
  • Western Blot 1:2000
• Theoretical MW: 30.2 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.3), 1.0% BSA and 50% Glycerol
• Preservative: 0.02% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for RASD2 Antibody (OTI1F7)

• GTP-binding protein Rhes
• MGC:4834
• RASD family, member 2
• Rhes
• TEM2Ras homolog enriched in striatum
• Tumor endothelial marker 2

Background

This gene encodes a Ras-related protein that enriched in striatum. The product of this gene binds to GTP and possesses intrinsic GTPase activity. The gene belongs to the Ras superfamily of small GTPases. The exact function of this gene is unknown, but most striatum-specific mRNAs characterized to date encode components of signal transduction cascades. [provided by RefSeq].

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for RASD2 Antibody (NBP2-03331)(1)

Publication using NBP2-03331

• Lu B, Palacino J. A novel human embryonic stem cell-derived Huntington's disease neuronal model exhibits mutant huntingtin (mHTT) aggregates and soluble mHTT-dependent neurodegeneration. FASEB J 2013-01-16 [PMID: 23325320] (WB, Human)

Bioinformatics

• Gene Symbol: RASD2
• Entrez:
  • 23551(Human)