Transgelin/TAGLN/SM22 alpha Antibody - BSA Free

Western Blot: Transgelin/TAGLN/SM22 alpha Antibody [NBP2-20405] - Sample (50 ug of whole cell lysate) A: Mouse Liver, 12% SDS PAGE gel, diluted at 1:1000.

Immunocytochemistry/Immunofluorescence: Transgelin/TAGLN/SM22 alpha Antibody [NBP2-20405] - HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: Transgelin protein stained by Transgelin antibody diluted at 1:100. Red: alpha Tubulin, a sytoskeleton marker, stained by alpha Tubulin antibody NBP2-43837) diluted at 1:500. Blue: Hoechst 33342 staining.

Immunohistochemistry: Transgelin/TAGLN/SM22 alpha Antibody [NBP2-20405] - Coronary artery vascular smooth muscle cells in fixed, frozen adult mouse heart. Primary antibody 1:100 dilution of a 0.2mg/ml solution. This image was submitted via customer Review.

Western Blot: Transgelin/TAGLN/SM22 alpha Antibody [NBP2-20405] - Western blot analysis of Transgelin and beta-actin (lower panel) Sample (35 ug of whole cell lysate) A: A-10 cell B: A-10 cell treated with MG132 NBP2-20405.

Immunohistochemistry-Paraffin: Transgelin/TAGLN/SM22 alpha Antibody [NBP2-20405] - Paraffin-embedded mouse heart. Transgelin antibody dilution: 1:500.

Immunohistochemistry-Paraffin: Transgelin/TAGLN/SM22 alpha Antibody [NBP2-20405] - Paraffin-embedded mouse esophagus. Transgelin antibody dilution: 1:500.

Immunohistochemistry-Paraffin: Transgelin/TAGLN/SM22 alpha Antibody [NBP2-20405] - 40X image of coronary artery vascular smooth muscle stained for transgelin. Primary Antibody dilution of 1:100. This image was submitted via customer Review.

Western Blot: Transgelin/TAGLN/SM22 alpha Antibody [NBP2-20405] - Various whole cell extracts (30 ug) were separated by 15% SDS-PAGE, and the membrane was blotted with Transgelin/TAGLN/SM22 alpha antibody (NBP2-20405) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Immunohistochemistry-Paraffin: Transgelin/TAGLN/SM22 alpha Antibody [NBP2-20405] - Transgelin/TAGLN/SM22 alpha antibody detects Transgelin/TAGLN/SM22 alpha protein at cytoplasm by immunohistochemical analysis.Sample: Paraffin-embedded mouse smooth muscle.Transgelin/TAGLN/SM22 alpha stained by Transgelin/TAGLN/SM22 alpha antibody (NBP2-20405) diluted at 1:500.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Western Blot: Transgelin/TAGLN/SM22 alpha Antibody [NBP2-20405] - Various whole cell extracts (30 ug) were separated by 15% SDS-PAGE, and the membrane was blotted with Transgelin antibody diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.

• Reactivity: Hu, Mu, Rt, Bv, Po
• Applications: WB, ICC/IF, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

Transgelin/TAGLN/SM22 alpha Antibody - BSA Free Summary

• Immunogen: Recombinant protein encompassing a sequence within the center region of human Transgelin/TAGLN/SM22 alpha. The exact sequence is proprietary.
• Localization: Cytoplasm
• Predicted Species: Porcine (98%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: TAGLN
• Purity: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:100-1:1000
  • Immunohistochemistry 1:100
  • Immunohistochemistry-Frozen
  • Immunohistochemistry-Paraffin 1:100-1:1000
  • Western Blot 1:500-1:10000
• Theoretical MW: 23 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Chicken (87%), Xenopus laevis (81%).

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7), 20% Glycerol
• Preservative: 0.025% Proclin 300
• Purity: Antigen Affinity-purified

Alternate Names for Transgelin/TAGLN/SM22 alpha Antibody - BSA Free

• DKFZp686P11128
• Protein WS3-10
• SM22 alpha
• SM2222 kDa actin-binding protein
• SMCCDKFZp686B01212
• Smooth muscle protein 22-alpha
• TAGLN
• TAGLN1
• transgelin variant 2
• Transgelin
• WS3-10
• WS3-10SM22-alpha

Background

The protein encoded by this gene is a transformation and shape-change sensitive actin cross-linking/gelling protein found in fibroblasts and smooth muscle. Its expression is down-regulated in many cell lines, and this down-regulation may be an early and sensitive marker for the onset of transformation. A functional role of this protein is unclear. Two transcript variants encoding the same protein have been found for this gene. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Reviews for Transgelin/TAGLN/SM22 alpha Antibody (NBP2-20405) (2)

Average Rating: 3

(Based on 2 reviews)

We have 2 reviews tested in 1 species: Mouse.

reviewed by: Verified Customer IHC Mouse 06/08/2017

Summary

• Application: Immunohistochemistry
• Sample Tested: Adult heart
• Species: Mouse
• Lot: CGVL0115111

Comments

• Comments: Sample was fixed overnight in 4% PFA, blocked with 1% BSA; 1.5% Normal donkey serum; 0.1% triton X for 1 hour, ON with primary antibody 1:100 dilution of a 0.2mg/ml solution, washed, stained 1 hour with gt antisheep antibody 1:500 concentration.

reviewed by: Michelle Tallquist IHC Mouse 05/03/2017

Summary

• Application: Immunohistochemistry
• Sample Tested: Adult heart
• Species: Mouse
• Lot: 41815

Comments

• Comments: Fixed o.n. 4% PFA, block 1 hour 1%BSA in 0.1% Triton/PBS, 1:100 dilution antibody in 0.1% Triton/PBS o.n., 1 hour secondary.

Bioinformatics

• Gene Symbol: TAGLN
• Uniprot:
  • Q01995()