Transgelin/TAGLN/SM22 alpha Antibody

Images

 
Western Blot: Transgelin/TAGLN/SM22 alpha Antibody [NBP1-33003] - Non-transfected (-) and transfected (+) HeLa whole cell extracts (15 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with Transgelin ...read more
Immunocytochemistry/ Immunofluorescence: Transgelin/TAGLN/SM22 alpha Antibody [NBP1-33003] - HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: Transgelin protein stained by Transgelin antibody ...read more
Immunohistochemistry-Paraffin: Transgelin/TAGLN/SM22 alpha Antibody [NBP1-33003] - Paraffin-embedded rat stomach Transgelin antibody diluted at 1:500.
Western Blot: Transgelin/TAGLN/SM22 alpha Antibody [NBP1-33003] - 30 ug C8D30 whole cell lysate/extract 12% SDS-PAGE gel, antibody dilution 1:1000.
Western Blot: Transgelin/TAGLN/SM22 alpha Antibody [NBP1-33003] - Sample (30 ug of whole cell lysate) A: Hep G2 12% SDS PAGE; antibody diluted at 1:1000.
Western Blot: Transgelin/TAGLN/SM22 alpha Antibody [NBP1-33003] - A. 30 ug Rat-2 whole cell lysate/extract 12 % SDS-PAGE Transgelin antibody dilution: 1:5000

Product Details

Summary
Reactivity Hu, Mu, Rt, Po, BvSpecies Glossary
Applications WB, ICC/IF, IHC, IHC-P, IP
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1 mg/ml
     

Genetic Strategies

   

Order Details

Transgelin/TAGLN/SM22 alpha Antibody Summary

Immunogen
Recombinant protein encompassing a sequence within the center region of human Transgelin. The exact sequence is proprietary.
Localization
Cytoplasm
Predicted Species
Porcine (98%), Bovine (97%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
TAGLN
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:1000-1:10000
  • Immunocytochemistry/Immunofluorescence 1:100-1:1000
  • Immunohistochemistry 1:100-1:1000
  • Immunohistochemistry-Paraffin 1:100-1:1000
  • Immunoprecipitation 1:100-1:500
Theoretical MW
23 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Publications
Read Publications using
NBP1-33003 in the following applications:

  • WB
    2 publications

Reactivity Notes

Expected cross reactivity based on sequence homology: Chicken (85%).

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
0.1M Tris (pH 7.0), 0.1M Glycine and 10% Glycerol
Preservative
0.01% Thimerosal
Concentration
1 mg/ml
Purity
Immunogen affinity purified

Alternate Names for Transgelin/TAGLN/SM22 alpha Antibody

  • DKFZp686P11128
  • Protein WS3-10
  • SM22 alpha
  • SM2222 kDa actin-binding protein
  • SMCCDKFZp686B01212
  • Smooth muscle protein 22-alpha
  • TAGLN
  • TAGLN1
  • transgelin variant 2
  • Transgelin
  • WS3-10
  • WS3-10SM22-alpha

Background

The protein encoded by this gene is a transformation and shape-change sensitive actin cross-linking/gelling protein found in fibroblasts and smooth muscle. Its expression is down-regulated in many cell lines, and this down-regulation may be an early and sensitive marker for the onset of transformation. A functional role of this protein is unclear. Two transcript variants encoding the same protein have been found for this gene. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Transgelin/TAGLN/SM22 alpha Antibody (NBP1-33003)(2)

We have publications tested in 2 confirmed species: Human, Mouse.

We have publications tested in 1 application: WB.


Filter By Application
WB
(2)
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Filter By Species
Human
(1)
Mouse
(1)
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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Secondary Antibodies

 

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Diseases for Transgelin/TAGLN/SM22 alpha Antibody (NBP1-33003)

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Bioinformatics

Gene Symbol TAGLN
Entrez
Uniprot