Transducin alpha Antibody

Images

 
Western Blot: Transducin alpha Antibody [NBP2-20696] - Mouse tissue extract (50 ug) was separated by 10% SDS-PAGE, and the membrane was blotted with GNAT1 antibody [N1C3] diluted at 1:3000.
Immunohistochemistry-Frozen: Transducin alpha Antibody [NBP2-20696] - Frozen sectioned adult mouse retina. Green: GNAT1 protein stained by GNAT1 antibody [N1C3] diluted at 1:250. Red: beta Tubulin 3/ TUJ1, stained by ...read more
Western Blot: Transducin alpha Antibody [NBP2-20696] - Sample (30 ug of whole cell lysate) A: U87-MG 10% SDS PAGE gel, diluted at 1:3000.

Product Details

Summary
Reactivity Hu, Mu, Rt, Bv, Ca, Ch, GP, Xp, ZeSpecies Glossary
Applications WB, IHC-Fr
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1 mg/ml

Order Details

Transducin alpha Antibody Summary

Immunogen
Recombinant protein encompassing a sequence within the center region of human GNAT1. The exact sequence is proprietary.
Predicted Species
Rat (98%), Chicken (96%), Canine (99%), Xenopus (92%), Bovine (98%), Guinea Pig (99%), Zebrafish (92%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
GNAT1
Purity
Immunogen affinity purified
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Applications/Dilutions

Dilutions
  • Western Blot 1:500-1:3000
  • Immunohistochemistry-Frozen 1:100-1:1000
Theoretical MW
40 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Xenopus laevis (92%).

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.0), 1.0% BSA and 20% Glycerol
Preservative
0.01% Thimerosal
Concentration
1 mg/ml
Purity
Immunogen affinity purified

Alternate Names for Transducin alpha Antibody

  • CSNBAD3
  • GBT1
  • GNATR
  • guanine nucleotide binding protein (G protein), alpha transducing activitypolypeptide 1
  • guanine nucleotide-binding protein G(t) subunit alpha-1
  • guanine nucleotide-binding protein G(T), alpha-1 subunit
  • rod-type transducin alpha subunit
  • Transducin alpha-1 chain
  • transducin, rod-specific

Background

Transducin is a 3-subunit guanine nucleotide-binding protein (G protein) which stimulates the coupling of rhodopsin and cGMP-phoshodiesterase during visual impulses. The transducin alpha subunits in rods and cones are encoded by separate genes. This gene encodes the alpha subunit in rods. This gene is also expressed in other cells, and has been implicated in bitter taste transduction in rat taste cells. Mutations in this gene result in autosomal dominant congenital stationary night blindness. Multiple alternatively spliced variants, encoding the same protein, have been identified. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Video Protocols

WB Video Protocol

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Secondary Antibodies

 

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Bioinformatics

Gene Symbol GNAT1
Uniprot