Melanopsin Antibody

Immunocytochemistry/Immunofluorescence: Melanopsin Antibody [NB100-74460] - Staining in rat retinal ganglion cells.

• Reactivity: Hu, Mu, Rt
• Applications: WB, ICC/IF, IHC, KO
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Concentration: 1 mg/ml

Melanopsin Antibody Summary

• Immunogen: Synthetic peptide corresponding to residues E(455) Q K S K T P K T K R H L P S L D R R M(474) of rat melanopsin.
• Localization: Membrane; Multi-pass membrane protein. Note: Found in soma, dendrites and proximal part of axons of certain retinal ganglion cells.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: OPN4
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 5 ug/mL
  • Immunohistochemistry 1:10 - 1:500
  • Immunohistochemistry-Frozen 1:1000
  • Knockout Validated
  • Western Blot 0.1 - 0.2 ug/mL
• Application Notes: Successful WB, IF, and ICC usages. WB: Detects approx. 53 and 85 kDa proteins representing overexpressed unglycosylated and glycosylated melanopsin from HEK 293 cells (does not work well on endogenously expressed protein). ICC/IF: NB100-74460 detects melanopsin from rat retinal ganglion cells (RGCs). Knockout data (PMID: 31924821).

Reactivity Notes

Human reactivity reported in scientific literature (PMID: 20971788). Mouse (PMID: 22886938).

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: PBS, 1 mg/mL BSA
• Preservative: 0.05% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for Melanopsin Antibody

• Melanopsin
• MGC142118
• MOP
• OPN4
• opsin 4
• Opsin-4

Background

Melanopsin is an opsin-like protein found in the retinal ganglion cells of mammals. Melanopsin is believed to be part of a secondary optical system that parallels that of the classic rod and cone system. This second system reports directly to the suprachiasmatic nucleus and is responsible for regulation of circadian rhythms. Melanopsin is believed to be the primary photopigment responsible for the regulation of these circadian rhythms, and Melanopsin knockout mice have been generated which demonstrate decreased capacity to entrain to light and dark cycles.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Melanopsin Antibody (NB100-74460)(2)

Publications using NB100-74460

• Platzl C, Kaser-Eichberger A, Trost A et al. Melanopsin in the human and chicken choroid Experimental eye research 2024-10-30 [PMID: 39151779]
• Roy D, Levi K, Kiss V et al. Rhodopsin and melanopsin coexist in mammalian sperm cells and activate different signaling pathways for thermotaxis Sci Rep 2020-01-10 [PMID: 31924821] (KO, ICC/IF, Mouse, Human)

Bioinformatics

• Gene Symbol: OPN4
• Entrez:
  • 30044(Mouse)
  • 192223(Rat)