Reactivity | Hu, Mu, RtSpecies Glossary |
Applications | WB, ICC/IF, IHC, IHC-Fr, KO |
Clonality | Polyclonal |
Host | Rabbit |
Conjugate | Unconjugated |
Concentration | 1 mg/ml |
Immunogen | Synthetic peptide corresponding to residues E(455) Q K S K T P K T K R H L P S L D R R M(474) of rat melanopsin. |
Localization | Membrane; Multi-pass membrane protein. Note: Found in soma, dendrites and proximal part of axons of certain retinal ganglion cells. |
Isotype | IgG |
Clonality | Polyclonal |
Host | Rabbit |
Gene | OPN4 |
Purity | Immunogen affinity purified |
Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
Dilutions |
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Application Notes | Successful WB, IF, and ICC usages. WB: Detects approx. 53 and 85 kDa proteins representing overexpressed unglycosylated and glycosylated melanopsin from HEK 293 cells (does not work well on endogenously expressed protein). ICC/IF: NB100-74460 detects melanopsin from rat retinal ganglion cells (RGCs). Knockout data (PMID: 31924821). |
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Publications |
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Storage | Store at -20C. Avoid freeze-thaw cycles. |
Buffer | PBS, 1 mg/mL BSA |
Preservative | 0.05% Sodium Azide |
Concentration | 1 mg/ml |
Purity | Immunogen affinity purified |
Publication using NB100-74460 | Applications | Species |
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Roy D, Levi K, Kiss V et al. Rhodopsin and melanopsin coexist in mammalian sperm cells and activate different signaling pathways for thermotaxis Sci Rep Jan 10 2020 [PMID: 31924821] (ICC/IF, KO, Human, Mouse) | ICC/IF, KO | Human, Mouse |
Secondary Antibodies |
Isotype Controls |
Diseases for Melanopsin Antibody (NB100-74460)Discover more about diseases related to Melanopsin Antibody (NB100-74460).
| Pathways for Melanopsin Antibody (NB100-74460)View related products by pathway.
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Research Areas for Melanopsin Antibody (NB100-74460)Find related products by research area.
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