Melanopsin Antibody

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Immunocytochemistry/ Immunofluorescence: Melanopsin Antibody [NB100-74460] - Staining in rat retinal ganglion cells.

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, ICC/IF, IHC, IHC-Fr, KO
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1 mg/ml

Order Details

Melanopsin Antibody Summary

Immunogen
Synthetic peptide corresponding to residues E(455) Q K S K T P K T K R H L P S L D R R M(474) of rat melanopsin.
Localization
Membrane; Multi-pass membrane protein. Note: Found in soma, dendrites and proximal part of axons of certain retinal ganglion cells.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
OPN4
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 0.1 - 0.2 ug/mL
  • Immunocytochemistry/Immunofluorescence 5 ug/mL
  • Immunohistochemistry 1:10 - 1:500
  • Immunohistochemistry-Frozen 1:1000
  • Knockout Validated
Application Notes
Successful WB, IF, and ICC usages. WB: Detects approx. 53 and 85 kDa proteins representing overexpressed unglycosylated and glycosylated melanopsin from HEK 293 cells (does not work well on endogenously expressed protein). ICC/IF: NB100-74460 detects melanopsin from rat retinal ganglion cells (RGCs). Knockout data (PMID: 31924821).
Publications
Read Publication using
NB100-74460 in the following applications:

Reactivity Notes

Human reactivity reported in scientific literature (PMID: 20971788). Mouse (PMID: 22886938).

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
PBS, 1 mg/mL BSA
Preservative
0.05% Sodium Azide
Concentration
1 mg/ml
Purity
Immunogen affinity purified

Alternate Names for Melanopsin Antibody

  • Melanopsin
  • MGC142118
  • MOP
  • OPN4
  • opsin 4
  • Opsin-4

Background

Melanopsin is an opsin-like protein found in the retinal ganglion cells of mammals. Melanopsin is believed to be part of a secondary optical system that parallels that of the classic rod and cone system. This second system reports directly to the suprachiasmatic nucleus and is responsible for regulation of circadian rhythms. Melanopsin is believed to be the primary photopigment responsible for the regulation of these circadian rhythms, and Melanopsin knockout mice have been generated which demonstrate decreased capacity to entrain to light and dark cycles.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Melanopsin Antibody (NB100-74460)(1)

We have publications tested in 2 confirmed species: Human, Mouse.

We have publications tested in 2 applications: ICC/IF, KO.


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ICC/IF
(1)
KO
(1)
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Mouse
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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Bioinformatics

Gene Symbol OPN4
Entrez