Transducin alpha Antibody

Images

 
Western Blot: Transducin alpha Antibody [NB120-3504] - Analysis of sheep retinal extracts.

Product Details

Summary
Reactivity Mu, Po, Bv, ShSpecies Glossary
Applications WB
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1 mg/ml

Order Details

Transducin alpha Antibody Summary

Immunogen
Synthetic Peptide: K(18) L K E D A E K D A R T V K(31) C
Localization
Cell membrane.
Specificity
Detects Rod Transducin alpha from bovine outer rod segments and sheep retinal extract.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
GNAT1
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1 ug/ml
Application Notes
WB: Detects an approx. 38 kDa protein representing Rod Transducin alpha from sheep retinal extract.

Reactivity Notes

Expected cross-reactivity with Human base on sequence homology. Mouse reactivity reported in scientific literature ( PMID: 11095744). Porcine reactivity reported in scientific literature ( PMID: 22108793).

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.05% Sodium Azide
Concentration
1 mg/ml
Purity
Immunogen affinity purified

Alternate Names for Transducin alpha Antibody

  • CSNBAD3
  • GBT1
  • GNATR
  • guanine nucleotide binding protein (G protein), alpha transducing activitypolypeptide 1
  • guanine nucleotide-binding protein G(t) subunit alpha-1
  • guanine nucleotide-binding protein G(T), alpha-1 subunit
  • rod-type transducin alpha subunit
  • Transducin alpha-1 chain
  • transducin, rod-specific

Background

Vision involves the conversion of light into electrochemical signals that are processed by the retina and subsequently sent to and interpreted by the brain. The process of converting light into an electrochemical signal begins when the membrane-bound protein, rhodopsin, absorbs light within the retina. Photoexcitation of rhodopsin causes the cytoplasmic surface of the protein to become catalytically active. In the active state, rhodopsin activates transducin, a GTP binding protein. Once activated, transducin promotes the hydrolysis of cGMP by phosphodiesterase (PDE). The decrease of intracellular cGMP concentration causes the ion channels within the outer segment of the rod or cone to close, thus causing membrane hyperpolarization and, eventually, signal transmission. Rhodopsin activity is believed to be shut off by phosphorylation followed by binding of the soluble protein, arrestin. Transducin, once activated by rhodopsin, promotes the hydrolysis of cGMP by PDE. The subunit composition of transducin differs between different photoreceptor cells. Rod transducin consists of rod transducin alpha (Tr alpha), T beta, and T gamma. Cone transducin is composed of cone transducin alpha (Tc alpha), T beta and T gamma. Differential transducin subunit composition of transducin is believed to be responsible for the different light sensitivities between photoreceptive cells.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customers Who Viewed This Item Also Viewed...

NBP1-47602
Species: Bv
Applications: WB, ELISA, ICC/IF, IHC, IHC-P, IP
NBL1-11162
Species: Hu
NB100-355
Species: Hu, Mu, Rt, Po, Bv, Ca, Ch, Xp
Applications: WB, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, CyTOF-ready
NB100-74460
Species: Hu, Mu, Rt
Applications: WB, ICC/IF, IHC, IHC-Fr
NBP2-01462
Species: Hu
Applications: WB, Flow, ICC/IF
NBL1-12154
Species: Hu
NBP2-50179
Species: Hu
Applications: WB, Flow, Func, ICC/IF, IP, CyTOF-ready
NBP2-60655
Species: Hu
Applications: WB, ICC/IF, IHC, IHC-P
NB120-5663
Species: Hu, Mu, Bv, Ca
Applications: WB, ICC/IF, IHC, IHC-Fr, IHC-P
NB120-2776
Species: Hu, Bv
Applications: WB, ICC/IF, IHC, IHC-Fr, IP
NBP1-46273
Species: Hu
Applications: WB, IHC, IHC-Fr, IHC-P
NBP1-88111
Species: Hu
Applications: WB, ICC/IF, IHC, IHC-P
NB110-41404
Species: Hu, Mu, Rt, Bv
Applications: WB, IHC
H00001406-M02
Species: Hu, Mu
Applications: WB, ChIP, ELISA, ICC/IF
PP-H7223-00
Species: Hu
Applications: WB, IHC, DirELISA
AF2945
Species: Hu
Applications: WB
NB120-3504
Species: Mu, Po, Bv, Sh
Applications: WB

Publications for Transducin alpha Antibody (NB120-3504) (0)

There are no publications for Transducin alpha Antibody (NB120-3504).
By submitting your publication information earn gift cards and discounts for future purchases.

Reviews for Transducin alpha Antibody (NB120-3504) (0)

There are no reviews for Transducin alpha Antibody (NB120-3504). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen
  • 1st to review with an image -- $50/€35/£30/$50 CAD/¥300 Yuan/¥5000 Yen

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for Transducin alpha Antibody (NB120-3504) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Secondary Antibodies

 

Isotype Controls

Additional Transducin alpha Products

Bioinformatics Tool for Transducin alpha Antibody (NB120-3504)

Discover related pathways, diseases and genes to Transducin alpha Antibody (NB120-3504). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
Visit Tool

Diseases for Transducin alpha Antibody (NB120-3504)

Discover more about diseases related to Transducin alpha Antibody (NB120-3504).
 

Pathways for Transducin alpha Antibody (NB120-3504)

View related products by pathway.

PTMs for Transducin alpha Antibody (NB120-3504)

Learn more about PTMs related to Transducin alpha Antibody (NB120-3504).
 

Research Areas for Transducin alpha Antibody (NB120-3504)

Find related products by research area.

Blogs on Transducin alpha

There are no specific blogs for Transducin alpha, but you can read our latest blog posts.

Customers Who Bought This Also Bought

Contact Information

Product PDFs

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Review this Product

Be the first to review our Transducin alpha Antibody and receive a gift card or discount.

Bioinformatics

Gene Symbol GNAT1
Entrez