Human TRAILR2/TNFRSF10B ELISA Kit (Colorimetric)

ELISA: Human TRAILR2/TNFRSF10B ELISA Kit (Colorimetric) [NBP3-14637] - Example of Human TRAILR2/TNFRSF10B Standard Curve.

• Reactivity: Hu
• Applications: ELISA
• Suitable Sample Type: Serum, Cell lysates, Cell culture supernatant
• Standard Curve Range: 31.25-1000 pg/ml
• Sensitivity: 6 pg/ml

Human TRAILR2/TNFRSF10B ELISA Kit (Colorimetric) Summary

• Description: Incubation: 2 hours 30 minutes
• Specificity: Specific for human TRAILR2/TNFRSF10B. No cross reactivity was observed with TRAIL, CD117, IL-6R, IL-2R, CD116, TRAIL R1, TRAIL R3, TRAIL R4, CD178 and Granzyme B..
• Standard Curve Range: 31.25-1000 pg/ml
• Sensitivity: 6 pg/ml
• Assay Type: Sandwich ELISA
• Inter-Assay: CV% < 7.4
• Intra-Assay: CV% < 3.2
• Spike Recovery: 83 to 124%
• Sample Volume: 100 ul
• Kit Type: ELISA Kit (Colorimetric)
• Gene: TNFRSF10B

Applications/Dilutions

• Dilutions:
  • ELISA

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.

Alternate Names for Human TRAILR2/TNFRSF10B ELISA Kit (Colorimetric)

• CD262 antigen
• CD262
• death domain containing receptor for TRAIL/Apo-2L
• Death receptor 5
• DR5
• DR5TRICK2B
• Fas-like protein
• KILLER/DR5
• KILLERapoptosis inducing protein TRICK2A/2B
• TNF-related apoptosis-inducing ligand receptor 2
• TNFRSF10B
• TRAIL R2
• TRAIL receptor 2
• TRAILR2
• TRAIL-R2apoptosis inducing receptor TRAIL-R2
• TRAILR2cytotoxic TRAIL receptor-2
• TRICK2
• TRICK2A
• TRICKB
• tumor necrosis factor receptor superfamily member 10B
• tumor necrosis factor receptor superfamily, member 10b
• tumor necrosis factor receptor-like protein ZTNFR9
• ZTNFR9

Background

Apoptosis is induced by certain cytokines including TNF and Fas ligand in the TNF family through their death domain containing receptors. TRAIL/Apo2L is a new member of the TNF family. DR4 was recently identified as the receptor for TRAIL. A novel death domain containing receptor for TRAIL was more recently identified and designated DR5, Apo2, TRAIL-R2, TRICK2, or KILLER by several groups independently (1-2). Like DR4, DR5 transcript is widely expressed in normal tissues and in many types of tumor cells. DR5 binds to TRAIL and mediates TRAIL induced cell death. Overexpression of DR5 induces apoptosis and activates NF-kB.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

FAQs for TRAILR2/TNFRSF10B ELISA Kit (NBP3-14637). (Showing 1 - 1 of 1 FAQ).

1. What is the difference between the Quantikine and Duoset ELISA kits?
   • Our Quantikine kits are fully optimized and validated for the sample types listed on the product specific webpage and datasheet, as this can vary between kits. Each kit is supplied ready to use with one pre-coated 96-well plate, a detection antibody directly conjugated to HRP, and all other necessary reagents. These kits are ideal for researchers who want the convenience of a ready to use and optimized ELISA product. Some of these kits are also available prepackaged in larger 6 and 50 plate sizes.Our DuoSet Kits, in contrast, are ELISA development kits containing the capture and detection antibody, the mass-value calibrated standard, and streptavidin-HRP to prepare approximately 5 or 15 plates. Ancillary reagents will need to be used/purchased, and for most kits, we will recommend one of our Ancillary Reagent Kits, which contain the reagents we use ourselves in-house. DuoSet kits are validated only for cell culture supernatant samples and therefore require further development and validation for accurate measurement in more complex samples such as serum and plasma. Our DuoSet Kits offer an economical, flexible alternative for the experienced ELISA user.

Bioinformatics

• Gene Symbol: TNFRSF10B