TRAF-4 Overexpression Lysate (Native)


Western Blot: TRAF4 Overexpression Lysate (Adult Normal) [NBL1-17244] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for TRAF4.

Product Details

Applications WB
The exact concentration of the protein of interest cannot be determined for overexpression lysates. Please contact technical support for more information.

Order Details

TRAF-4 Overexpression Lysate (Native) Summary

The lysate was created in HEK293T cells, using plasmid ID RC200345 and based on accession number NM_004295. The protein contains a C-terminal DDK tag.
Homo sapiens TNF receptor-associated factor 4 (TRAF4), mRNA.

Packaging, Storage & Formulations

Store at -80C. Avoid freeze-thaw cycles.
RIPA buffer
The exact concentration of the protein of interest cannot be determined for overexpression lysates. Please contact technical support for more information.


Application Notes
This product is intended for use as a positive control in Western Blot. You will receive the lysate (100ug), and an empty vector negative control (100 ug).
Theoretical MW
53.4 kDa

Lysate Details for TRAF-4

Protein State


HEK293T cells in 10-cm dishes were transiently transfected with a non-lipid polymer transfection reagent specially designed and manufactured for large volume DNA transfection. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4, and then centrifuged to clarify the lysate. Protein concentration was measured by BCA protein assay kit.This product is manufactured by and sold under license from OriGene Technologies and its use is limited solely for research purposes.

Alternate Names for TRAF-4 Overexpression Lysate (Native)

  • CART1
  • CART1RING finger protein 83
  • Cysteine-rich domain associated with RING and Traf domains protein 1
  • Metastatic lymph node gene 62 protein
  • MLN 62
  • MLN62
  • MLN62cysteine-rich domain associated with ring and TRAF domain
  • RNF83malignant 62
  • TNF receptor-associated factor 4
  • TRAF4
  • tumor necrosis receptor-associated factor 4A


This gene encodes a member of the TNF receptor associated factor (TRAF) family. TRAF proteins are associated with, and mediate signal transduction from members of the TNF receptor superfamily. The encoded protein has been shown to interact with neurotrophin receptor, p75 (NTR/NTSR1), and negatively regulate NTR induced cell death and NF-kappa B activation. This protein has been found to bind to p47phox, a cytosolic regulatory factor included in a multi-protein complex known as NAD(P)H oxidase. This protein thus, is thought to be involved in the oxidative activation of MAPK8/JNK. Alternatively spliced transcript variants have been observed but the full-length nature of only one has been determined. [provided by RefSeq]. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications.


This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

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Publications for TRAF-4 Lysate (NBL1-17244) (0)

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Video Protocols

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Contact Information

Product PDFs


Gene Symbol TRAF4

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