TRADD Antibody (313203) [Unconjugated]

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TRADD was detected in immersion fixed MCF‑7 human breast cancer cell line using Mouse Anti-Human TRADD Monoclonal Antibody (Catalog # MAB2658) at 3 µg/mL for 3 hours at room temperature. Cells were stained ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications CyTOF-ready, Flow, ICC/IF
Clone
313203
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
LYOPH

TRADD Antibody (313203) [Unconjugated] Summary

Immunogen
E. coli-derived recombinant human TRADD
Met1-Ala312
Accession # Q15628
Specificity
Detects human TRADD in direct ELISAs and Western blots. In Western blots, no cross-reactivity with  recombinant human (rh) BAFF R, rhTNF R1, rhHVEM, rhLT beta R, rhCD30, rhGITR, rh4-1BB, rhDR3, rhDR6, rhRANK, rhRELT, rhTAJ, rhEDAR, rhFas, recombinant mouse (rm) DC‑TRAIL R1, rmDC-TRAIL R2, rhTRAIL R3, rhTRAIL R4, rhNGF R, rhCD27, rhTNF RII, rhTWEAK R, rhTRAIL R2, or rhCD40 is observed.
Details of Functionality
ActivityAssaywCitation not found None None
Source
N/A
Isotype
IgG2a
Clonality
Monoclonal
Host
Mouse
Gene
TRADD
Endotoxin Note
<0.10 EU per 1 μg of the antibody by the LAL method.
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • CyTOF-ready
  • Immunocytochemistry 3-25 ug/mL
  • Intracellular Staining by Flow Cytometry 0.25 ug/10^6 cells
Publications
Read Publication using
MAB2658 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for TRADD Antibody (313203) [Unconjugated]

  • MGC11078
  • TNFRSF1A-associated via death domainTNFR1-associated DEATH domain protein
  • TRADD
  • tumor necrosis factor receptor type 1 associated death domain protein
  • tumor necrosis factor receptor type 1-associated DEATH domain protein
  • tumor necrosis factor receptor-1-associated protein

Background

TRADD (TNF R1A-associated death domain protein) is a 34 kDa protein that is expressed nearly ubiquitously. TRADD functions as an adaptor protein associating with the cytoplasmic domain of TNF receptor 1 or to another adaptor, FADD (Fas-interacting DD) to form the DISC (death inducing signaling complex). Human TRADD is an alpha-helical, Greek key, death domain-containing protein that is 77% identical to both mouse and rat TRADD.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for TRADD Antibody (MAB2658)(1)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 1 application: Western Blot.


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(1)
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Blogs on TRADD.

Caspase 9: The Suicidal Cell Whisperer
Cell death via apoptosis is a key cellular function triggered by the cell death receptor family and their ligands which signal through downstream adaptor molecules and the caspase protease family. Among the subclass of initiator caspases that include...  Read full blog post.

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Bioinformatics

Gene Symbol TRADD
Uniprot