TRADD Antibody (313203) [Unconjugated]

TRADD was detected in immersion fixed MCF-7 human breast cancer cell line using Mouse Anti-Human TRADD Monoclonal Antibody (Catalog # MAB2658) at 3 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

• Reactivity: Hu
• Applications: CyTOF-ready, Flow, ICC/IF
• Clone: 313203
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: LYOPH

TRADD Antibody (313203) [Unconjugated] Summary

• Immunogen: E. coli-derived recombinant human TRADD
  Met1-Ala312
  Accession # Q15628
• Specificity: Detects human TRADD in direct ELISAs and Western blots. In Western blots, no cross-reactivity with recombinant human (rh) BAFF R, rhTNF R1, rhHVEM, rhLT beta R, rhCD30, rhGITR, rh4-1BB, rhDR3, rhDR6, rhRANK, rhRELT, rhTAJ, rhEDAR, rhFas, recombinant mouse (rm) DC‑TRAIL R1, rmDC-TRAIL R2, rhTRAIL R3, rhTRAIL R4, rhNGF R, rhCD27, rhTNF RII, rhTWEAK R, rhTRAIL R2, or rhCD40 is observed.
• Source: N/A
• Isotype: IgG2a
• Clonality: Monoclonal
• Host: Mouse
• Gene: TRADD
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • CyTOF-ready
  • Immunocytochemistry 3-25 ug/mL
  • Intracellular Staining by Flow Cytometry 0.25 ug/10^6 cells

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for TRADD Antibody (313203) [Unconjugated]

• MGC11078
• TNFRSF1A-associated via death domainTNFR1-associated DEATH domain protein
• TRADD
• tumor necrosis factor receptor type 1 associated death domain protein
• tumor necrosis factor receptor type 1-associated DEATH domain protein
• tumor necrosis factor receptor-1-associated protein

Background

TRADD (TNF R1A-associated death domain protein) is a 34 kDa protein that is expressed nearly ubiquitously. TRADD functions as an adaptor protein associating with the cytoplasmic domain of TNF receptor 1 or to another adaptor, FADD (Fas-interacting DD) to form the DISC (death inducing signaling complex). Human TRADD is an alpha-helical, Greek key, death domain-containing protein that is 77% identical to both mouse and rat TRADD.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for TRADD Antibody (MAB2658)(1)

Publication using MAB2658

• Kolliputi N, Waxman AB IL-6 cytoprotection in hyperoxic acute lung injury occurs via suppressor of cytokine signaling-1-induced apoptosis signal-regulating kinase-1 degradation. Am. J. Respir. Cell Mol. Biol., 2008-09-05;40(3):314-24. 2008-09-05 [PMID: 18776134] (Western Blot, Human)

Bioinformatics

• Gene Symbol: TRADD
• Uniprot:
  • Q15628()