TR4/NR2C2 Antibody (OTI4B1)

Western Blot: TR4/NR2C2 Antibody (4B1) [NBP2-46228] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY NR2C2.

Immunohistochemistry: TR4/NR2C2 Antibody (4B1) [NBP2-46228] - Analysis of Human tonsil tissue. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120C for 3 min)

Immunohistochemistry: TR4/NR2C2 Antibody (4B1) [NBP2-46228] - Analysis of Human breast tissue.(Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120C for 3 min)

Immunohistochemistry: TR4/NR2C2 Antibody (4B1) [NBP2-46228] - Analysis of Human colon tissue. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120C for 3 min)

Immunohistochemistry: TR4/NR2C2 Antibody (4B1) [NBP2-46228] - Analysis of Adenocarcinoma of Human colon tissue. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120C for 3 min)

Immunohistochemistry: TR4/NR2C2 Antibody (4B1) [NBP2-46228] - Analysis of Human Kidney tissue. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120C for 3 min)

Immunohistochemistry: TR4/NR2C2 Antibody (4B1) [NBP2-46228] - Analysis of Carcinoma of Human kidney tissue. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120C for 3 min)

Immunohistochemistry: TR4/NR2C2 Antibody (4B1) [NBP2-46228] - Analysis of Human liver tissue. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120C for 3 min)

Immunohistochemistry: TR4/NR2C2 Antibody (4B1) [NBP2-46228] - Analysis of Carcinoma of Human liver tissue.(Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120C for 3 min)

Immunohistochemistry: TR4/NR2C2 Antibody (4B1) [NBP2-46228] - Analysis of Human Ovary tissue. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120C for 3 min)

Immunohistochemistry: TR4/NR2C2 Antibody (4B1) [NBP2-46228] - Analysis of Adenocarcinoma of Human ovary tissue. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120C for 3 min)

Immunohistochemistry: TR4/NR2C2 Antibody (4B1) [NBP2-46228] - Analysis of Human pancreas tissue. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120C for 3 min)

Immunohistochemistry: TR4/NR2C2 Antibody (4B1) [NBP2-46228] - Analysis of Human thyroid tissue. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120C for 3 min)

Immunohistochemistry: TR4/NR2C2 Antibody (4B1) [NBP2-46228] - Analysis of Carcinoma of Human thyroid tissue. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120C for 3 min)

Immunohistochemistry: TR4/NR2C2 Antibody (4B1) [NBP2-46228] - Analysis of Human endometrium. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120C for 3 min)

Immunohistochemistry: TR4/NR2C2 Antibody (4B1) [NBP2-46228] - Analysis of Adenocarcinoma of Human endometrium tissue. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120C for 3 min)

Immunohistochemistry: TR4/NR2C2 Antibody (4B1) [NBP2-46228] - Analysis of Human prostate tissue. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120C for 3 min)

Immunohistochemistry: TR4/NR2C2 Antibody (4B1) [NBP2-46228] - Analysis of Carcinoma of Human prostate tissue. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120C for 3 min)

Immunohistochemistry: TR4/NR2C2 Antibody (4B1) [NBP2-46228] - Analysis of Human bladder tissue. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120C for 3 min)

Immunohistochemistry: TR4/NR2C2 Antibody (4B1) [NBP2-46228] - Analysis of Human lymph node tissue. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120C for 3 min)

Immunohistochemistry: TR4/NR2C2 Antibody (4B1) [NBP2-46228] - Analysis of Human lymphoma tissue. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120C for 3 min)

• Reactivity: Hu, Mu, Rt
• Applications: WB, IHC
• Clone: OTI4B1
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: 1 mg/ml

TR4/NR2C2 Antibody (OTI4B1) Summary

• Description: Novus Biologicals Mouse TR4/NR2C2 Antibody (OTI4B1) (NBP2-46228) is a monoclonal antibody validated for use in IHC and WB. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Human recombinant protein fragment corresponding to amino acids 1-241 of human NR2C2(NP_003289) produced in E.coli.
• Isotype: IgG2b
• Clonality: Monoclonal
• Host: Mouse
• Gene: NR2C2
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 1:150
  • Immunohistochemistry-Paraffin
  • Western Blot 1:2000
• Theoretical MW: 67.2 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.3), 1.0% BSA and 50% Glycerol
• Preservative: 0.02% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for TR4/NR2C2 Antibody (OTI4B1)

• hTAK1
• NR2C2
• Nuclear hormone receptor TR4
• nuclear receptor subfamily 2 group C member 2
• nuclear receptor subfamily 2, group C, member 2
• Orphan nuclear receptor TAK1
• Orphan nuclear receptor TR4
• TAK1
• TAK1TR4TR2R1
• Testicular receptor 4
• TR4 nuclear hormone receptor
• TR4

Background

TR4 (TAK1, NR2C2) is an orphan nuclear receptor. TR4 was originally cloned from lymphoblastoma Raji cells or mouse brain cDNA library. No ligand has been reported. Northern blot shows TR4 is transcribed as a 9kb mRNA in many tissues and as a 2.8kb mRNA in testis, mainly in spermatocytes. TR4 has two isoforms called TR4alpha1 and TR4 alpha2,which differ in 19 amino acids coded by two separate exons. Both products translated from the 9kb transcript are ubiquitously expressed. Since TR4 binds to the same elements for the RAR-RXR or TR-RXR heterodimers, TR4 may have an inhibitory effect for retinoic-acid mediated transactivation.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: NR2C2