TR2/NR2C1 Antibody (OTI4C7)

Western Blot: TR2/NR2C1 Antibody (4C7) [NBP2-46227] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY NR2C1.

Immunohistochemistry: TR2/NR2C1 Antibody (4C7) [NBP2-46227] - Analysis of Adenocarcinoma of Human breast tissue.

• Reactivity: Hu, Mu, Rt
• Applications: WB, IHC
• Clone: OTI4C7
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: 1 mg/ml

TR2/NR2C1 Antibody (OTI4C7) Summary

• Description: Novus Biologicals Mouse TR2/NR2C1 Antibody (OTI4C7) (NBP2-46227) is a monoclonal antibody validated for use in IHC and WB. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Human recombinant protein fragment corresponding to amino acids 1-237 of human NR2C1 (NP_001027458) produced in E.coli.
• Isotype: IgG2a
• Clonality: Monoclonal
• Host: Mouse
• Gene: NR2C1
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 1:150
  • Immunohistochemistry-Paraffin
  • Western Blot 1:2000
• Theoretical MW: 51 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.3), 1.0% BSA and 50% Glycerol
• Preservative: 0.02% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for TR2/NR2C1 Antibody (OTI4C7)

• NR2C1
• nuclear receptor subfamily 2 group C member 1
• nuclear receptor subfamily 2, group C isoform
• nuclear receptor subfamily 2, group C, member 1
• Orphan nuclear receptor TR2
• Testicular receptor 2
• TR2 nuclear hormone receptor
• TR2
• TR2-11

Background

NR2C1 encodes a nuclear hormone receptor characterized by a highly conserved DNA binding domain (DBD), a variable hinge region, and a carboxy-terminal ligand binding domain (LBD) that is typical for all members of the steroid/thyroid hormone receptor superfamily. This protein also belongs to a large family of ligand-inducible transcription factors that regulate gene expression by binding to specific DNA sequences within promoters of target genes. Multiple alternatively spliced transcript variants have been described, but the full-length nature of some of these variants has not been determined. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: NR2C1