TPT1/TCTP Antibody (JU13-32)

Western Blot: TPT1/TCTP Antibody (JU13-32) [NBP2-75686] - Analysis of TCTP on different lysates using anti-TCTP antibody at 1/1,000 dilution.Positive control:Lane 1: Mouse placenta tissue Lane 2: Mouse testis tissueLane 3: Hela Lane 4: A431 Lane 5: MCF-7

Immunocytochemistry/Immunofluorescence: TPT1/TCTP Antibody (JU13-32) [NBP2-75686] - Staining TCTP in SH-SY5Y cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Immunohistochemistry-Paraffin: TPT1/TCTP Antibody (JU13-32) [NBP2-75686] - Analysis of paraffin-embedded mouse testis tissue using anti-TCTP antibody. Counter stained with hematoxylin.

Flow Cytometry: TPT1/TCTP Antibody (JU13-32) [NBP2-75686] - Analysis of THP-1 cells with TCTP antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor® 488-conjugated goat anti-rabbit IgG was used as the secondary antibody.

Immunocytochemistry/Immunofluorescence: TPT1/TCTP Antibody (JU13-32) [NBP2-75686] - Staining TCTP in A431 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Immunocytochemistry/Immunofluorescence: TPT1/TCTP Antibody (JU13-32) [NBP2-75686] - Staining TCTP in LOVO cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Immunohistochemistry-Paraffin: TPT1/TCTP Antibody (JU13-32) [NBP2-75686] - Analysis of paraffin-embedded human colon cancer tissue using anti-TCTP antibody. Counter stained with hematoxylin.

Product Discontinued

TPT1/TCTP Antibody (JU13-32) Summary

• Additional Information: Recombinant Monoclonal Antibody.
• Immunogen: Synthetic peptide within Human TPT1/TCTP aa 35-84 / 172. (SwissProt: P13693 Human; SwissProt: P63028 Mouse; SwissProt: P63029 Rat)
• Localization: Cytoplasm.
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Gene: TPT1
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 1:50-1:100
  • Immunocytochemistry/ Immunofluorescence 1:50-1:200
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 1:50-1:200
  • Immunoprecipitation
  • Western Blot 1:500-1:2000
• Theoretical MW: 20 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: TBS (pH7.4), 0.05% BSA, 40% Glycerol
• Preservative: 0.05% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Protein A purified

Alternate Names for TPT1/TCTP Antibody (JU13-32)

• FLJ27337
• Fortilin
• Histamine-releasing factor
• HRF
• p02
• p23
• TCTP
• TCTPHRFfortilin
• TPT1
• translationally-controlled tumor protein
• tumor protein, translationally-controlled 1

Background

Histamine is an inflammatory mediator that is ubiquitously expressed and has a broad range of pharmacologic effects. Specifically, it plays a role in the central nervous, gastrointestinal, respiratory and immune systems. Histamine release is mediated by the stimulation of mast cells and basophils. Histamine-releasing factor (HRF) is a cytokine-like molecule that causes the release of histamine, IL-4 and IL-13 from basophils as well as the secretion of IL-8 and a calcium response in eosinophils. HRF belongs to the translationally controlled tumor protein (TCTP) family and is detected as a protein of 21 kDa in mice and 23 kDa in human. It is expressed in several healthy and tumoral cells, including erythrocytes, hepatocytes, macrophages, platelets, keratinocytes, erythroleukemia cells, gliomas, melanomas, hepatoblastomas and lymphomas, and it is localized in the cytoplasm. HRF plays a pivotal role in allergic diseases, and due to its wide distribution in brain, is thought to be involved in neurodegenerative disorders, such as Alzheimer's disease and Down Syndrome.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: TPT1