TPT1/TCTP Antibody (488411) [Unconjugated]

Western blot shows lysates of Raji human Burkitt's lymphoma cell line, LNCaP human prostate cancer cell line, Y3-Ag rat myeloid cell line, and C2C12 mouse myoblast cell line. PVDF membrane was probed with 1 µg/mL Mouse Anti-Human/Mouse/Rat TPT1/TCTP Monoclonal Antibody (Catalog # MAB4648) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). For additional reference, recombinant human TPT1 (5 ng) was included. A specific band for TPT1/TCTP was detected at approximately 23 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 3.

TPT1/TCTP was detected in immersion fixed HEL 92.1.7 human erythroleukemic cell line using Mouse Anti-Human/Mouse/Rat TPT1/TCTP Monoclonal Antibody (Catalog # MAB4648) at 3 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Non-adherent Cells.

• Reactivity: Hu, Mu, Rt
• Applications: WB, ICC/IF
• Clone: 488411
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: LYOPH

TPT1/TCTP Antibody (488411) [Unconjugated] Summary

• Immunogen: E. coli-derived recombinant human TPT1/TCTP
  Met1-Cys172
  Accession # P13693
• Specificity: Detects human, mouse, and rat TPT1/TCTP in Western blots.
• Source: N/A
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: TPT1
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry 3-25 ug/mL
  • Western Blot 1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for TPT1/TCTP Antibody (488411) [Unconjugated]

• FLJ27337
• Fortilin
• Histamine-releasing factor
• HRF
• p02
• p23
• TCTP
• TCTPHRFfortilin
• TPT1
• translationally-controlled tumor protein
• tumor protein, translationally-controlled 1

Background

TPT1 (translationally controlled tumor protein 1; also TCTP, HRF and fortilin) is a 23 kDa member of the TCTP family of calcium‑binding proteins. It is widely expressed (but not in kidney), and apparently serves as a transcriptional activator, calcium transporter, histamine inducer and antiapoptotic, caspase-3 inhibitor. Human TPT1 is 172 amino acids (aa) in length, with multiple potential splice variants. There is an alternate start site at Met35 that may be accompanied by a deletion of aa 99‑172. In addition, there may be either a 25 aa or 16 aa C-terminal extension after Cys172. Over aa 1‑172, human TPT1 is 96% aa identical to mouse TPT1.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: TPT1
• Uniprot:
  • P13693()