TPRKB Antibody (OTI3H3) - Azide and BSA Free

Western Blot: TPRKB Antibody (OTI3H3) - Azide and BSA Free [NBP2-74574] - Analysis of extracts (35ug) from 9 different cell lines by using g anti-TPRKB monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).

Immunocytochemistry/Immunofluorescence: TPRKB Antibody (OTI3H3) - Azide and BSA Free [NBP2-74574] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY TPRKB.

Immunohistochemistry: TPRKB Antibody (OTI3H3) - Azide and BSA Free [NBP2-74574] - Staining of paraffin-embedded Human pancreas tissue using anti-TPRKB mouse monoclonal antibody.

Flow Cytometry: TPRKB Antibody (OTI3H3) - Azide and BSA Free [NBP2-74574] - HEK293T cells transfected with either overexpression plasmid (Red) or empty vector control plasmid (Blue) were immunostaining by anti-TPRKB antibody, and then analyzed by flow cytometry.

Immunohistochemistry: TPRKB Antibody (OTI3H3) - Azide and BSA Free [NBP2-74574] - Staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-TPRKB mouse monoclonal antibody.

Immunohistochemistry: TPRKB Antibody (OTI3H3) - Azide and BSA Free [NBP2-74574] - Staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-TPRKB mouse monoclonal antibody.

Immunohistochemistry: TPRKB Antibody (OTI3H3) - Azide and BSA Free [NBP2-74574] - Staining of paraffin-embedded Carcinoma of Human bladder tissue using anti-TPRKB mouse monoclonal antibody.

Immunohistochemistry: TPRKB Antibody (OTI3H3) - Azide and BSA Free [NBP2-74574] - Staining of paraffin-embedded Carcinoma of Human lung tissue using anti-TPRKB mouse monoclonal antibody.

Immunohistochemistry: TPRKB Antibody (OTI3H3) - Azide and BSA Free [NBP2-74574] - Staining of paraffin-embedded Carcinoma of Human pancreas tissue using anti-TPRKB mouse monoclonal antibody.

Immunohistochemistry: TPRKB Antibody (OTI3H3) - Azide and BSA Free [NBP2-74574] - Staining of paraffin-embedded Carcinoma of Human thyroid tissue using anti-TPRKB mouse monoclonal antibody.

Immunohistochemistry: TPRKB Antibody (OTI3H3) - Azide and BSA Free [NBP2-74574] - Staining of paraffin-embedded Human colon tissue using anti-TPRKB mouse monoclonal antibody.

Immunohistochemistry: TPRKB Antibody (OTI3H3) - Azide and BSA Free [NBP2-74574] - Staining of paraffin-embedded Human Kidney tissue using anti-TPRKB mouse monoclonal antibody.

Immunohistochemistry: TPRKB Antibody (OTI3H3) - Azide and BSA Free [NBP2-74574] - Staining of paraffin-embedded Human liver tissue using anti-TPRKB mouse monoclonal antibody.

Immunohistochemistry: TPRKB Antibody (OTI3H3) - Azide and BSA Free [NBP2-74574] - Staining of paraffin-embedded Human lymphoma tissue using anti-TPRKB mouse monoclonal antibody.

Immunohistochemistry: TPRKB Antibody (OTI3H3) - Azide and BSA Free [NBP2-74574] - Staining of paraffin-embedded Human tonsil using anti-TPRKB mouse monoclonal antibody.

• Reactivity: Hu, Mu, Pm, Pm
• Applications: WB, Flow, ICC/IF, IHC, CyTOF-ready
• Clone: OTI3H3
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free
• Concentration: LYOPH

TPRKB Antibody (OTI3H3) - Azide and BSA Free Summary

• Description: Novus Biologicals Mouse TPRKB Antibody (OTI3H3) - Azide and BSA Free (NBP2-03885) is a monoclonal antibody validated for use in IHC, WB, Flow and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Full length human recombinant protein of human TPRKB (NP_057142) produced in E.coli.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: TPRKB
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • CyTOF-ready
  • Flow Cytometry 1:100
  • Immunocytochemistry/ Immunofluorescence 1:100
  • Immunohistochemistry 1:150
  • Immunohistochemistry-Paraffin
  • Western Blot 1:500-1000
• Theoretical MW: 19.5 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Lyophilized from PBS (pH 7.3) with 8% Trehalose
• Preservative: No Preservative
• Concentration: LYOPH
• Purity: Immunogen affinity purified
• Reconstitution Instructions: we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process.

Alternate Names for TPRKB Antibody (OTI3H3) - Azide and BSA Free

• CGI-121
• PRPK (p53-related protein kinase)-binding protein
• PRPK-binding protein
• TP53RK binding protein
• TP53RK-binding protein

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: TPRKB