TPMT Antibody (OTI2A2) - Azide and BSA Free

Western Blot: TPMT Antibody (OTI2A2) - Azide and BSA Free [NBP2-74570] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY TPMT (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-TPMT.

Immunocytochemistry/Immunofluorescence: TPMT Antibody (OTI2A2) - Azide and BSA Free [NBP2-74570] - Staining of HepG2 cells using anti-TPMT mouse monoclonal antibody.

Immunohistochemistry: TPMT Antibody (OTI2A2) - Azide and BSA Free [NBP2-74570] - Staining of paraffin-embedded colon tissue using anti-TPMT mouse monoclonal antibody.

Immunohistochemistry: TPMT Antibody (OTI2A2) - Azide and BSA Free [NBP2-74570] - Staining of paraffin-embedded Adenocarcinoma of colon tissue using anti-TPMT mouse monoclonal antibody.

Immunohistochemistry: TPMT Antibody (OTI2A2) - Azide and BSA Free [NBP2-74570] - Staining of paraffin-embedded Adenocarcinoma of endometrium tissue using anti-TPMT mouse monoclonal antibody.

Immunohistochemistry: TPMT Antibody (OTI2A2) - Azide and BSA Free [NBP2-74570] - Staining of paraffin-embedded Carcinoma of liver tissue using anti-TPMT mouse monoclonal antibody.

Immunohistochemistry: TPMT Antibody (OTI2A2) - Azide and BSA Free [NBP2-74570] - Staining of paraffin-embedded Carcinoma of thyroid tissue using anti-TPMT mouse monoclonal antibody.

Immunohistochemistry: TPMT Antibody (OTI2A2) - Azide and BSA Free [NBP2-74570] - Staining of paraffin-embedded liver tissue using anti-TPMT mouse monoclonal antibody.

• Reactivity: Hu
• Applications: WB, ICC/IF, IHC
• Clone: OTI2A2
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free
• Concentration: LYOPH

TPMT Antibody (OTI2A2) - Azide and BSA Free Summary

• Description: Novus Biologicals Mouse TPMT Antibody (OTI2A2) - Azide and BSA Free (NBP2-02356) is a monoclonal antibody validated for use in IHC, WB and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Full length human recombinant protein of human TPMT (NP_000358) produced in HEK293T cell.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: TPMT
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:100
  • Immunohistochemistry 1:50
  • Immunohistochemistry-Paraffin
  • Western Blot 1:1000
• Theoretical MW: 28.2 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Lyophilized from PBS (pH 7.3) with 8% Trehalose
• Preservative: No Preservative
• Concentration: LYOPH
• Purity: Immunogen affinity purified
• Reconstitution Instructions: we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process.

Alternate Names for TPMT Antibody (OTI2A2) - Azide and BSA Free

• EC 2.1.1.67
• S-adenosyl-L-methionine:thiopurine S-methyltransferase
• Thiopurine methyltransferase
• thiopurine S-methyltransferase
• TPMT

Background

TPMT encodes the enzyme that metabolizes thiopurine drugs via S-adenosyl-L-methionine as the S-methyl donor and S-adenosyl-L-homocysteine as a byproduct. Thiopurine drugs such as 6-mercaptopurine are used as chemotherapeutic agents. Genetic polymorphisms that affect this enzymatic activity are correlated with variations in sensitivity and toxicity to such drugs within individuals. A pseudogene for this locus is located on chromosome 18q.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: TPMT