TOP2B Antibody [Unconjugated]

Western blot shows lysates of K562 human chronic myelogenous leukemia cell line. Gels were loaded with 30 µg of whole cell lysate (WCL), 20 µg of cytoplasmic (Cyto), and 10 µg of nuclear extracts (Nuc). PVDF Membrane was probed with 0.2 µg/mL of Sheep Anti-Human TOP2B Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6348) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for TOP2B at approximately 185 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Western blot shows lysates of HEK293 human embryonic kidney parental cell line and TOP2B knockout HEK293 cell line (KO). PVDF membrane was probed with 0.2 µg/mL of Sheep Anti-Human TOP2B Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6348) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for TOP2B at approximately 245 kDa (as indicated) in the parental HEK293 cell line, but is not detectable in knockoutHEK293 cell line. GAPDH (Catalog # AF5718) is shown as a loading control. This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

• Reactivity: Hu
• Applications: WB, KO
• Clonality: Polyclonal
• Host: Sheep
• Conjugate: Unconjugated
• Concentration: LYOPH

TOP2B Antibody [Unconjugated] Summary

• Immunogen: E. coli-derived recombinant human TOP2B
  aa 1187-1621
  Accession # Q02880
• Specificity: Detects human TOP2B in Western blots.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Sheep
• Gene: TOP2B
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Western Blot 0.2 ug/mL
  • Knockout Validated
• Control:

  TOP2B Knockout 293T Cell Lysate

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for TOP2B Antibody [Unconjugated]

• antigen MLAA-44
• DNA topoisomerase 2-beta
• DNA topoisomerase II beta
• DNA topoisomerase II, 180 kD
• DNA topoisomerase II, beta isozyme
• EC 5.99.1.3
• TOP2B
• top2beta
• TOPIIB
• topo II beta
• topoisomerase (DNA) II beta (180kD)
• topoisomerase (DNA) II beta 180kDa
• topoisomerase II beta
• topoisomerase IIb
• U937 associated antigen

Background

TOP2B (DNA Topoisomerase II beta ) is a 180‑185 kDa member of the type IIA subfamily, topoisomerase family of molecules. It is ubiquitously expressed, and represents the larger of two known topoisomerases (the 2A/ alpha -form being 170 kDa in size). TOP2A is essential for cell division, while TOP2B is active postmitotically. In an ATP-dependent manner, homodimeric TOP2B relieves torsional stress created in DNA during transcription, or as a consequence of replication. TOP2B first induces cleavage of double-stranded DNA, creating a space that allows for the physical repositioning of chromatin and a reduction in tension. This is followed by closure and ligation of the cleaved ends to recreate the original DNA structure. Human TOP2B is 1626 amino acids (aa) in length. It contains an ATPase domain (aa 101‑201), an NES (aa 1034‑1044), more that 30 Ser/Thr phosphorylation sites and a C-terminal DTHCT region (aa 1508‑1611). There is one splice variant that shows a deletion of aa 24‑28. Over aa 1187‑1621, human TOP2B shares 91% aa identity with mouse TOP2B.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: TOP2B
• Uniprot:
  • Q02880()