TOP2A Antibody (TOP2A/1361)

Western Blot: TOP2A Antibody (TOP2A/1361) [NBP2-47643] - HepG2, HeLa and 3T3 Cell Lysate using Topo II alpha, Monoclonal Antibody (TOP2A/1361).

Immunohistochemistry-Paraffin: TOP2A Antibody (TOP2A/1361) [NBP2-47643] - Formalin-fixed, paraffin-embedded Human Bladder Carcinoma stained with Topoisomerase II alpha Monoclonal Antibody (TOP2A/1361).

Immunohistochemistry-Paraffin: TOP2A Antibody (TOP2A/1361) [NBP2-47643] - Formalin-fixed, paraffin-embedded Human Tonsil stained with Topoisomerase II alpha Monoclonal Antibody (TOP2A/1361).

Product Discontinued

TOP2A Antibody (TOP2A/1361) Summary

• Immunogen: A recombinant human Topoisomerase II alpha fragment (aa1352-1493)
• Localization: Nuclear
• Marker: Proliferation & Drug-Resistance Marker
• Specificity: It recognizes a 170kDa protein, which is identified as topoisomerase IIa. It shows no cross-reaction with Topoisomerase IIb or I. Topo IIa plays important roles in synthesis and transcription of DNA as well as chromosomal segregation during mitosis. It is reported to be a sensitive and specific marker of late S-, G2- & M-phases in transformed and developmentally regulated normal cells. Topo IIa is also implicated in drug resistance of tumor cells.
• Isotype: IgG2b Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: TOP2A
• Purity: Protein A or G purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 0.5-1ug/million cells
  • Immunofluorescence 1-2ug/ml
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 0.5-1ug/ml
  • Western Blot 0.5-1ug/ml
• Application Notes: Human Chromosome Location: 17q21.2
  Immunohistology (Formalin-fixed) (0.5-1ug/ml for 30 min at RT) (Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Citrate Buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes)
• Theoretical MW: 170 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C.
• Buffer: 10 mM PBS with 0.05% BSA
• Preservative: 0.05% Sodium Azide
• Concentration: 0.2 mg/ml
• Purity: Protein A or G purified

Alternate Names for TOP2A Antibody (TOP2A/1361)

• DNA topoisomerase (ATP-hydrolyzing)
• DNA topoisomerase 2-alpha
• DNA topoisomerase II, 170 kD
• DNA topoisomerase II, alpha isozyme
• EC 5.99.1.3
• TOP2
• TOP2A
• TOP2DNA gyrase
• topoisomerase (DNA) II alpha (170kD)
• topoisomerase (DNA) II alpha 170kDa
• TP2A

Background

Topo II Alpha encodes a DNA topoisomerase, an enzyme that controls and alters the topologic states of DNA during transcription. This nuclear enzyme is involved in processes such as chromosome condensation, chromatid separation, and the relief of torsional stress that occurs during DNA transcription and replication. It catalyzes the transient breaking and rejoining of two strands of duplex DNA which allows the strands to pass through one another, thus altering the topology of DNA. Two forms of this enzyme exist as likely products of a gene duplication event. The gene encoding this form, alpha, is localized to chromsome 17 and the beta gene is localized to chromosome 3. The gene encoding this enzyme functions as the target for several anticancer agents and a variety of mutations in this gene have been associated with the development of drug resistance. Reduced activity of this enzyme may also play a role in ataxia-telangiectasia.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: TOP2A